Untargeted metabolomics analysis reveals the biochemical variations of polyphenols in a diverse apple population

Q4 Agricultural and Biological Sciences

Fruit Growing Research Pub Date : 2023-01-01 DOI:10.48130/frures-2023-0029

Jun Song, Beatrice Amyotte, Cindy H.J. Yu, Leslie Campbell Palmer, Melinda Vinqvist-Tymchuk, H.P. Vasantha Rupasinghe

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Abstract

Apples are an important dietary source of polyphenols as antioxidants that contribute to human health. Based on total phenolic content and total antioxidant capacity data gathered from a large apple biodiversity collection, we applied untargeted metabolomics analyses to further understand the phenolic composition in select accessions with very low and very high phenolic contents, as well as in commercial cultivars. The liquid chromatography-mass spectrometry (LC-MS) analysis provided both qualitative and quantitative information, with 2,946 features detected using the positive mode of electrospray ionization. We found significant variation in total polyphenols, and strong correlations between total phenolic content and total antioxidant capacity. Polyphenolic compounds significantly associated with each group of apples were quantified, and new features were putatively identified. This study provides new knowledge regarding the specific polyphenols that contribute to the variation in total phenolic content and antioxidant capacity among apples, and new insights into the biochemical regulations of polyphenol biosynthesis and composition.

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非靶向代谢组学分析揭示了不同苹果群体中多酚的生化变化

苹果是多酚类抗氧化剂的重要膳食来源，对人体健康有益。基于大量苹果生物多样性资料收集的总酚含量和总抗氧化能力数据，我们应用非靶向代谢组学分析进一步了解酚含量极低和极高的精选品种以及商品品种的酚组成。液相色谱-质谱(LC-MS)分析提供了定性和定量信息，使用电喷雾电离的正模式检测了2,946个特征。我们发现总多酚含量存在显著差异，总多酚含量与总抗氧化能力之间存在很强的相关性。对每组苹果中显著相关的多酚化合物进行了量化，并推测出了新的特征。本研究对苹果总酚含量和抗氧化能力变化的具体多酚类物质有了新的认识，对多酚生物合成和组成的生化调控有了新的认识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fruit Growing Research Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

0.10

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： FRUIT GROWING RESEARCH is an international journal, which has been issued under the care of the Research Institute for Fruit Growing Pitesti, Romania. Fruit growing research is an annual journal aimed at disseminating significant research and original papers on genetic resources, breeding of deciduous fruit trees species; fruit sustainable technologies – environment protection; propagation, virology and tissue culture, postharvest, that can be of interest to a wide audience of plant scientists in all areas of fruit growing. An international Editorial Board advises the journal.