Genetic profile of HIV-1 in the Republic Sakha, Yakutia

Q4 Medicine

HIV Infection and Immunosuppressive Disorders Pub Date : 2023-10-21 DOI:10.22328/2077-9828-2023-15-3-73-83

V. O. Kotova, O. E. Trotsenko, L. A. Balakhontseva, E. A. Bazykina, L. S. Sokolova, V. N. Kulagina, R. N. Fedorova

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Abstract

Aim. To perform molecular-genetic analysis of HIV-1 variants circulation in the Republic Sakha (Yakutia) and analysis of drug resistance mutations. Materials and methods. 123 patients diagnosed with HIV infection were examined. Mutations of drug resistance were detected via sequencing of amplified fragments of pol gene that is coding protease and a part of reverse transcriptase of HIV-1 using «Amplisens® HIV-Resist-Seq» test-system. Phylogenetic analysis was performed with software MEGA version 7.0. Results and discussion . Sub-subtype A6 that is prevalent in the Russian Federation was revealed most frequently (78.0%) in the examined group. Subtype B was detected in four cases (3.3%). Isolated cases of infection due to subtypes C, G and subsubtype A7 were revealed. Recombinant forms of the virus were found in 16.2% of the patients (n=20). Drug resistance mutations were revealed in 65 patients (52.8%) that were undergoing antiretroviral therapy (ART) which dictates a necessity of changing ART drugs. Conclusion . The results of the research state changes in the genetic profile of HIV-1 variants that were circulating during the last years in the Republic Sakha (Yakutia).

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雅库特萨哈共和国HIV-1基因图谱

的目标。在萨哈共和国(雅库特)进行HIV-1变异循环的分子遗传学分析和耐药性突变分析。材料和方法。对123例诊断为HIV感染的患者进行了检查。使用«Amplisens®hiv - resistance - seq»测试系统，通过对编码蛋白酶和部分HIV-1逆转录酶的pol基因扩增片段进行测序，检测耐药突变。采用MEGA 7.0软件进行系统发育分析。结果和讨论。俄罗斯联邦流行的A6亚型在检查组中最常见(78.0%)。B亚型4例(3.3%)。C、G、A7亚型感染病例均有分离。在16.2%的患者(n=20)中发现病毒的重组形式。在接受抗逆转录病毒治疗(ART)的患者中，有65例(52.8%)发现耐药突变，这表明有必要更换抗逆转录病毒药物。结论。研究结果表明，过去几年在萨哈共和国(雅库特)流行的HIV-1变异基因谱发生了变化。

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来源期刊

HIV Infection and Immunosuppressive Disorders Medicine-Infectious Diseases

CiteScore

0.70

自引率

0.00%

发文量

期刊介绍： In the scientific-practical journal "HIV Infection and Immunosuppressive Disorders", published various issues of HIV medicine (epidemiology, molecular mechanisms of pathogenesis to the development of educational programs) leading scientists of Russia and countries of CIS, USA, as well as practical healthcare professionals working in research centers, research institutes, universities, clinics where done basic medical work. A special place on the pages of the publication is given to basic and clinical research, analytical reviews of contemporary and foreign reports, the provision of medical care for various diseases.