COMPARATIVE ANTI-INFLAMMATORY STUDIES ON TIMOLOL MALEATE AND DICLOFENAC SODIUM IN, IN VITRO AND IN VIVO MODELS BY ORAL ROUTES

Abstract

The present study was conducted on timolol maleate (a non-selective beta-blocker) using in vitro assays and in vivo models of rats. It was tested for its anti-inflammatory activity at three dose levels (1.028 mg kg-1 , 5.14 mg kg -1 and 10.28 mg kg -1). Furthermore, the investigation was supported by the estimation of tumor necrosis factor-alpha (tNF-α), interleukin-1 (IL- 1) and histopathological examination. It was observed that at 1.028 mg kg -1, 5.14 mg kg -1 and 10.28 mg kg -1 the drug showed anti-inflammatory activities. moreover, plasma levels of tNF-α and IL-1 showed inhibition. Histopathological examination confirmed the highest anti-inflammatory activity at 5.14 mg kg -1. Thus, the conducted studies revealed that timolol maleate, when given orally does possesses an anti-inflammatory potential.