Expression and purification of recombinant thermostable DNA-binding protein <i>Sso7d</i>

Abstract

The Sso7d protein has exceptional structural stability and the ability to bind highly specifically to DNA, which makes the protein a promising module for creating fusion proteins and test systems. Sso7d is a part of fusion high-fidelity DNA polymerases capable of carrying out the polymerase chain reaction even in the presence of PCR inhibitors. Application of faster, simpler, and more efficient method for protein production will significantly reduce the cost of creating biosensors and conducting analyzes. This paper describes a new efficient method for obtaining recombinant Sso7d protein with a high degree of purity without using affinity chromatography.