Validation of an Aqueous Normal Phase Chromatography Method for the Analysis of Ergothioneine in Commercial Mushrooms

IF 0.7 4区化学 Q4 CHEMISTRY, ANALYTICAL

Lc Gc North America Pub Date : 2023-09-01 DOI:10.56530/lcgc.na.zi2474s4

Joseph J. Pesek, Maria T. Matyska, Tanya Hiltz, Gary Takeoka

引用次数: 0

Abstract

An analytical HPLC method for the identification and quantification of ergothioneine (EGT) has been developed using a silica hydride-based column. A simplified sample preparation method was developed. Several types of commercially available mushrooms were analyzed for quantification of ergothioneine using a Cogent Diamond Hydride column (100 x 4.6 mm) at a flow rate of 1 mL/min, with a deionized water:acetonitrile:0.1% formic acid mobile phase using an aqueous normal phase (ANP) gradient. EGT was detected at 254 nm and was identified by matching retention times of the verified standard. The standard ergothioneine content ranged from 0.01 mg to 0.1 mg/mL with excellent linearity correlation (R2 = 0.99999). EGT recoveries were within 96.5–100.3%. The developed HPLC method was simple, accurate, reproducible, sensitive and rugged.

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正相色谱法分析商品蘑菇中麦角硫因的验证

建立了麦角硫因(EGT)的高效液相色谱鉴别和定量方法。建立了一种简化的样品制备方法。使用Cogent Diamond hydr化物色谱柱(100 x 4.6 mm)，流速为1ml /min，以去离子水:乙腈:0.1%甲酸为流动相，使用水正相(ANP)梯度，分析几种类型的市售蘑菇，以定量测定麦角硫因。在254 nm处检测EGT，并通过匹配验证标准品的保留时间进行鉴定。麦角硫因标准含量范围为0.01 ~ 0.1 mg/mL，线性关系良好(R2 = 0.99999)。EGT加样回收率在96.5 ~ 100.3%之间。该方法简便、准确、重现性好、灵敏度高、耐用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Lc Gc North America 化学-分析化学

CiteScore

1.00

自引率

55.60%

发文量

审稿时长

2 months

期刊介绍： Founded in 1983, LCGC is the leading provider of digital and print content to the separation science market, enhancing the productivity, efficiency, and the overall value of separation techniques globally. Founded in 1983, LCGC is the leading provider of digital and print content to the separation science market, enhancing the productivity, efficiency, and the overall value of separation techniques globally. With our commitment to editorial excellence we have pioneered innovation across a broad portfolio of digital and print platforms.