The Antibacterial Potency of Moringa oleifera Leaf Extract on Semau Island, East Nusa Tenggara, Against Escherichia coli

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McGill International Journal of Sustainable Development Law and Policy Pub Date : 2023-10-23 DOI:10.55908/sdgs.v11i10.591

None Kusmiyati, Ferry WF Waangsir, Moses Kopong Tokan, Apris A Adu, Anwar Mallongi, Eryc Zevrily Haba Bunga

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Abstract

Objective: This study was conducted to investigate the phytochemical constituent of Moringa oleifera leaf extract and determine the inhibition of these extract against Escherichia coli bacteria. Methodology: This was a laboratory experimental study. Moringa leaves was collected from Semau Island, East Nusa tenggara, Indonesia. Quantitative testing was carried out to determine the bioactive components of Moringa leaf extract. The well diffusion method was used to test the inhibitory activity of moringa leaf extract against E. coli. The treatment were divided into 10 extract concentrations (10%, 20%, 30%, 40%, 50%, 60%,70%, 80%, 90%, 100%). Results: This study showed the presence of flavonoids, phenols, alkaloids, tannins and saponins in M. oleifera leaf extract. M. oleifera leaf extract has different inhibitory properties against E. coli bacteria. Moringa extract concentrations of 10%, 20% and did not show inhibitory activity against E. coli while the others concentrations were able to inhibit the growth of E. coli. The average inhibition zone diameter for each concentration varied i.e 1.91 mm (40%), 6.27 mm (50%), 8.53mm (60%), 10.39 mm (70%), 12.80 mm (80%), 14.84 mm (90%) and 19.04 mm (100%). The most effective for inhibiting the growth of E. coli bacteria was 100%. The results of statistical analysis using repeated ANOVA tests obtained p value 0.001 (p<0.05) that means the M. oleifera leaf extract able to inhibit E. coli. Contributions: We conclude that M. oleifera leaves have the potential as an antibacterial, so further studies related to the use of M. oleifera in overcoming various environmental health problems is needed.

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东努沙登加拉岛辣木叶提取物对大肠杆菌的抑菌作用

目的:研究辣木叶提取物的植物化学成分，并测定其对大肠杆菌的抑制作用。方法:本研究为实验室实验研究。辣木叶采自印度尼西亚东努沙登加拉岛的Semau岛。对辣木叶提取物的生物活性成分进行了定量测定。采用孔扩散法测定辣木叶提取物对大肠杆菌的抑菌活性。将处理分为10个提取浓度(10%、20%、30%、40%、50%、60%、70%、80%、90%、100%)。结果:油松叶提取物中含有黄酮类化合物、酚类化合物、生物碱、单宁和皂苷。油橄榄叶提取物对大肠杆菌具有不同的抑制作用。10%、20%辣木提取物浓度对大肠杆菌无抑制作用，其余浓度对大肠杆菌均有抑制作用。各浓度的平均抑菌带直径分别为1.91 mm(40%)、6.27 mm(50%)、8.53mm(60%)、10.39 mm(70%)、12.80 mm(80%)、14.84 mm(90%)和19.04 mm(100%)。对大肠杆菌生长的抑制效果为100%。使用重复方差分析进行统计分析的结果得到p值0.001 (p<0.05)，这意味着油橄榄叶提取物能够抑制大肠杆菌。结论:油桐叶具有潜在的抗菌作用，需要进一步研究油桐在克服各种环境健康问题中的应用。

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McGill International Journal of Sustainable Development Law and Policy LAW-

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