Characterization and Antimicrobial Susceptibility Pattern of Non-fermenting Gram-negative Bacilli and Molecular Analysis of Pseudomonas Species from Respiratory Samples of Intensive Care Unit Patients in A Tertiary Care Hospital

IF 0.7 Q4 MICROBIOLOGY
Umamakeshwari Dhanalakshmi Jeyaraman, Padmakumari Jagadeesan, Vinotha Sundaram, Vanaja Rebba
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引用次数: 0

Abstract

Among patients admitted to the intensive care unit (ICU) the most common cause of infection is lower respiratory tract infections, which account for 4.4% of hospital admissions. Among the non-fermenters, Pseudomonas aeruginosa and Acinetobacter calcoaceticus-baumannii complex, cause many types of nosocomial infections. Ventilator-associated pneumonia is the most common emerging infection among them. The goal of this study was to isolate and speciate non-fermenting Gram-negative organisms from respiratory samples of ICU patients and to detect antimicrobial susceptibility patterns of the isolated non-fermenters. This cross-sectional study was conducted at the Institute of Microbiology in collaboration with Medical Intensive Care units in Rajiv Gandhi Government General Hospital. A total of 200 patients who satisfied the inclusion criteria were included in the study conducted between March 2019 and March 2020. Culture, sensitivity, and anti-microbial susceptibility tests were performed for the respiratory samples collected as per standard protocols. Pseudomonas aeruginosa (49%) was the most commonly isolated non-fermenter followed by Acinetobacter baumannii (24.3%). Among Pseudomonas aeruginosa isolates, the highest percentage were ESBL producers (44.4%). Carbapenem resistance among Pseudomonas isolates was 33%. The study showed increased isolation of MDR non-fermenters from the ICU causing Ventilator-associated pneumonia (VAP). To prevent VAP caused by these MDR pathogens, clinicians should follow strict infection control practices, use invasive devices on a short-term basis, and use antibiotics judiciously.
某三级医院重症监护病人呼吸样本非发酵革兰氏阴性杆菌的鉴定、药敏模式及假单胞菌的分子分析
在入住重症监护病房(ICU)的患者中,最常见的感染原因是下呼吸道感染,占住院人数的4.4%。在非发酵菌中,铜绿假单胞菌和钙酸-鲍曼复合不动杆菌引起多种类型的医院感染。呼吸机相关性肺炎是其中最常见的新发感染。本研究的目的是从ICU患者的呼吸道样本中分离和鉴定非发酵革兰氏阴性菌,并检测分离的非发酵革兰氏阴性菌的抗菌药敏模式。这项横断面研究是在微生物研究所与拉吉夫·甘地政府总医院的医疗重症监护室合作进行的。该研究于2019年3月至2020年3月期间进行,共有200名符合纳入标准的患者被纳入研究。按照标准方案对采集的呼吸道样本进行培养、敏感性和抗微生物药敏试验。铜绿假单胞菌(49%)是最常见的非发酵菌,其次是鲍曼不动杆菌(24.3%)。在铜绿假单胞菌分离株中,ESBL产生菌的比例最高(44.4%)。假单胞菌对碳青霉烯的耐药率为33%。该研究表明,从ICU中分离出的引起呼吸机相关性肺炎(VAP)的耐多药非发酵菌增加。为了预防由这些耐多药病原体引起的VAP,临床医生应遵循严格的感染控制措施,在短期内使用侵入性设备,并明智地使用抗生素。
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来源期刊
Journal of Pure and Applied Microbiology
Journal of Pure and Applied Microbiology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
2.00
自引率
0.00%
发文量
266
审稿时长
11 months
期刊介绍: Journal of Pure and Applied Microbiology (JPAM) is a peer-reviewed, open access international journal of microbiology aims to advance and disseminate research among scientists, academics, clinicians and microbiologists around the world. JPAM publishes high-quality research in all aspects of microbiology in both online and print form on quarterly basis.
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