Crude extract and effective fractions from Andrographis paniculata for immature Aedes aegypti and Culex quinquefasciatus mosquito

IF 4.7 Q1 ECOLOGY

生态学报 Pub Date : 2023-11-01 DOI:10.1016/j.chnaes.2023.10.005

Jelin Vilvest, M.C. John Milton, Alex Yagoo

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引用次数: 0

Abstract

In the quest for environmentally friendly alternatives to combat the challenges of resistance development and environmental toxicity associated with synthetic insecticides in mosquito control, the aim of this study is to explore the potential of fractions derived from Andrographis paniculata as environmentally friendly alternatives for controlling Aedes aegypti and Culex quinquefasciatus mosquitoes, with a focus on assessing their larvicidal, pupicidal, and ovicidal activities. Initially, crude extracts of hexane, chloroform, and methanol from A. paniculata leaves were evaluated for their efficacy against Ae. aegypti and Cx. quinquefasciatus mosquitoes, with the methanol extract showing promising activity. Subsequently, a comprehensive methanol extraction of A. paniculata leaves was conducted, followed by fractionation via column chromatography using various eluents, including chloroform, ethyl acetate, methanol, and their combinations. This process yielded 80 fractions, which were consolidated into 10 fractions based on thin-layer chromatography (TLC) profiles. Each fraction was assessed for ovicidal, larvicidal, and pupicidal activity across concentrations of 2.5, 5.0, 7.5, and 10 ppm. Among these fractions, ‘F-4’ exhibited exceptional efficacy against the larvae, pupae, and eggs of both mosquito species. At a concentration of 10 ppm, ‘F-4’ achieved 100% larval mortality, with LC50 values of 7.2 ppm for Ae. aegypti and 5.0 ppm for Cx. quinquefasciatus. Additionally, ‘F-4’ demonstrated substantial pupicidal activity, with LC50 values of 7.6 ppm for Ae. aegypti and 5.7 ppm for Cx. quinquefasciatus. Furthermore, ‘F-4’ exhibited ovicidal activity, with efficacy ranging between 77% and 78%. These results highlight ‘F-4’ from A. paniculata shows promise as an eco-friendly biocontrol agent against Ae. aegypti and Cx. quinquefasciatus mosquitoes, warranting further investigation for sustainable mosquito control.

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穿心莲粗提物及其对未成熟埃及伊蚊和致倦库蚊的有效部位

为了寻找环境友好型替代品，以应对蚊虫控制中与合成杀虫剂相关的抗性发展和环境毒性挑战，本研究的目的是探索穿心莲提取物作为环境友好型替代品的潜力，以控制埃及伊蚊和致倦库蚊，重点评估其杀幼虫、杀蛹和杀卵活性。首先，研究了金针叶中己烷、氯仿和甲醇粗提物对伊蚊的抑制作用。埃及伊蚊和Cx。甲醇提取物对致倦库蚊具有良好的活性。随后，采用氯仿、乙酸乙酯、甲醇及其组合等多种洗脱剂，对金针叶进行综合甲醇提取，并进行柱层析分离。该工艺得到80个馏分，根据薄层色谱(TLC)图谱，这些馏分被合并成10个馏分。在浓度分别为2.5、5.0、7.5和10ppm时，对每个馏分的杀卵、杀幼虫和杀蛹活性进行评估。在这些组分中，“F-4”对两种蚊子的幼虫、蛹和卵都表现出特殊的功效。在浓度为10 ppm时，F-4的幼虫死亡率达到100%，对伊蚊的LC50值为7.2 ppm。埃及伊蚊和5.0 ppm Cx。quinquefasciatus。此外，F-4对伊蚊的LC50值为7.6 ppm，具有很强的杀毒活性。埃及伊蚊和5.7 ppm的Cx。quinquefasciatus。此外，“F-4”表现出杀卵活性，效力范围在77%至78%之间。这些结果表明，A. paniculata中的F-4作为一种生态友好型的生物防治剂具有良好的应用前景。埃及伊蚊和Cx。致倦库蚊，需要进一步研究以实现蚊虫的可持续控制。

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来源期刊

生态学报 Environmental Science-Ecology

CiteScore

5.30

自引率

0.00%

发文量

17028

审稿时长

68 days

期刊介绍： Our Journal publishes recent theories and novel experimental results in ecology, and facilitates academic exchange and discussions both domestically and abroad. It is expected that our journal will promote the development of and foster research talents for ecological studies in China.