Dioscin from Polygonatum sibiricum induces apoptosis and autophagy in Ishikawa human endometrial cancer cell and in vivo

IF 5.6 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
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Abstract

With an aim to comprehend the precise regulatory mechanism of dioscin against endometrial carcinoma (EC), we firstly extracted the components from Polygonatum sibiricum followed by identification and structural characterization. The anti-EC activity of dioscin was initially determined based on the inhibition of Ishikawa cell proliferation and tumor growth. The high-throughput sequencing data indicated that dioscin not only promoted apoptosis, including decrease of poly ADP-ribose polymerase (PARP) and B-cell lymphoma-2 (Bcl-2) and increase of c-PARP and Bcl-2-associcated agonist of cell death (Bad), but also induced autophagy, including increase of autophagic lysosomes and LC3II/LC3Ⅰ ratio. Mechanistic exploration suggested that dioscin induced autophagy and apoptosis through inhibition of PI3K/AKT/mTOR signaling pathway. Besides, the dioscin-regulated p53 pathway was mainly involved in autophagy induction. Furthermore, inhibition of Ishikawa cell autophagy was linked to dioscin-induced apoptosis. Our data suggest the immense potential of dioscin for the development of functional food for EC and related medical application.

Abstract Image

何首乌中的薯蓣皂苷可诱导石川人子宫内膜癌细胞和体内细胞凋亡和自噬
为了理解地奥司钦对子宫内膜癌(EC)的精确调控机制,我们首先从西伯利亚何首乌中提取了成分,然后进行了鉴定和结构表征。根据对石川细胞增殖和肿瘤生长的抑制作用,初步确定了薯蓣皂苷的抗子宫内膜癌活性。高通量测序数据表明,薯蓣皂苷不仅能促进细胞凋亡,包括减少多聚 ADP 核糖聚合酶(PARP)和 B 细胞淋巴瘤-2(Bcl-2),增加 c-PARP 和 Bcl-2 细胞死亡激动剂(Bad),还能诱导自噬,包括增加自噬溶酶体和 LC3II/LC3Ⅰ 比值。机理研究表明,地奥辛通过抑制PI3K/AKT/mTOR信号通路诱导自噬和细胞凋亡。此外,地奥司钦调控的p53通路主要参与自噬诱导。此外,石川细胞自噬的抑制与 dioscin 诱导的细胞凋亡有关。我们的数据表明,薯蓣皂苷在开发欧共体功能食品和相关医疗应用方面具有巨大潜力。
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来源期刊
Food Science and Human Wellness
Food Science and Human Wellness Agricultural and Biological Sciences-Food Science
CiteScore
8.30
自引率
5.70%
发文量
80
审稿时长
28 days
期刊介绍: Food Science and Human Wellness is an international peer-reviewed journal that provides a forum for the dissemination of the latest scientific results in food science, nutriology, immunology and cross-field research. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. By their effort, it has been developed to promote the public awareness on diet, advocate healthy diet, reduce the harm caused by unreasonable dietary habit, and directs healthy food development for food industrial producers.
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