Efficacy of Teucrium polium leaves extract as anticoccidial and anthelmintic: In vitro study

IF 0.4 4区农林科学 Q4 VETERINARY SCIENCES

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia Pub Date : 2023-10-01 DOI:10.1590/1678-4162-13014

E.M. Al-Shaebi, S. Al-Quraishy, R. Abdel-Gaber, S.N. Maodaa, A. Alatawi, S.A. Alawwad

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Abstract

ABSTRACT Natural sources are microbiological species and medicinal plants, which could be potential new sources for development of drugs against different diseases. Coccidiosis affects many animals and leads to great economic losses. Drug-resistant strains of Eimeria species have emerged because of overuse and misuse of drugs. In vitro, using Eimeria papillata oocyst and earthworm (Eisenia fetida), we evaluated the anticoccidial and anthelmintic effect of Teucrium polium leaves extract (TPLE). Using infrared spectroscopy showed the presence of thirteen compounds for TPLE. Mebendazole (10 mg/mL) caused paralysis and earthworm death by 13.91±0.373 and 18.2±0.980 min, respectively, while, for TPLE (100 mg/ml) were 4.23±0.077 and 4.817±0.386 min. Also, the histological study revealed obvious surface architecture abnormality for treated worms. Moreover, TPLE (300 mg/mL) and formalin (5%) at 72, 96, and 120 hrs led to inhibition of sporulation by approximately 100% with marked deformities, while TPLE (200, 100, 50, and 25 mg/mL), amprolium, DettolTM, and phenol at 120 hr approximately 65.9%, 23.6%, 4.8%, 3.2%, 12.6%, 68.4%, and 46.6%, respectively. This pilot investigation revealed that TPLE possesses anticoccidial and anthelmintic activity, encouraging additional testing in vivo to create a new medication for the treatment of coccidiosis and helminthiasis.

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枸杞叶提取物抗球虫、驱虫作用的体外研究

摘要天然资源是指微生物物种和药用植物，是开发各种疾病药物的潜在新资源。球虫病影响许多动物，造成巨大的经济损失。由于药物的过度使用和误用，艾美耳球虫品种出现了耐药菌株。在体外以乳突艾美耳球虫卵囊和蚯蚓为实验材料，研究了茯苓叶提取物(TPLE)的抗球虫和驱虫作用。红外光谱分析表明，TPLE中存在13个化合物。甲苯达唑(10 mg/mL)对蚯蚓的麻痹和死亡时间分别为13.91±0.373和18.2±0.980 min，而TPLE (100 mg/mL)对蚯蚓的麻痹和死亡时间分别为4.23±0.077和4.817±0.386 min。此外，TPLE (300 mg/mL)和福尔马林(5%)在72、96和120小时对孢子的抑制率约为100%，并有明显的畸形，而TPLE(200、100、50和25 mg/mL)、氨苯铵、德妥尔tm和苯酚在120小时对孢子的抑制率分别为65.9%、23.6%、4.8%、3.2%、12.6%、68.4%和46.6%。这项试点调查显示，TPLE具有抗球虫和驱虫药活性，鼓励进一步的体内试验，以创造一种新的治疗球虫病和寄生虫病的药物。

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来源期刊

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia 农林科学-兽医学

CiteScore

0.80

自引率

25.00%

发文量

111

审稿时长

9-18 weeks

期刊介绍： Publica artigos originais de pesquisa sobre temas de medicina veterinária, zootecnia, tecnologia e inspeção de produtos de origem animal e áreas afins relacionadas com a produção animal. Atualmente a revista mantém 628 permutas (419 internacionais e 209 nacionais), sendo um verdadeiro suporte para o recebimento de periódicos pela Biblioteca da Escola. A partir de 1999, a Escola de Veterinária delegou à FEP MVZ Editora o encargo do gerenciamento e edição de todas suas publicações, inclusive do Arquivo, ficando somente com o apoio logístico (instalações, equipamentos, pessoal etc.). O apoio financeiro é exercido pelo CNPq/FINEP e pela própria FEP MVZ.