Selection and isolation of bacterium with probiotic potential from the Amazon ornamental fish Hypancistrus sp. (Siluriformes, Loricariidae)

Pub Date : 2023-11-01 DOI:10.20950/1678-2305/bip.2023.49.e743

Ryuller Gama Abreu Reis, Higo Andrade Abe, Natalino da Costa Sousa, Rossineide Martins da Rocha

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Abstract

The aim of this study was to select autochthonous bacteria with probiotic potential from the gastrointestinal tract of King Tiger Pleco (Hypancistrus sp.) for future applications in captive production. For the isolation of lactic acid bacteria, 12 specimens (8.31 ± 0.28 cm and 12.60 ± 1.13 cm) were used, whose intestines were removed, macerated, and homogenized in sterile saline. A total of 21 strains were isolated from the intestinal tract on MRS agar, of which five strains (St1, St2, St3, St4, and St5) were selected for in-vitro tests. Three strains (St2, St3, and St5) showed the highest values of maximum growth per hour, with a final concentration of 109 CFU.mL-1 in 24 hours. However, only the strains St3 and St5 had the highest growth (p<0.05) in the presence of NaCl (0.5 to 1.5%) and pH in the range of 5 and 6. For bile salts, the greatest resistance observed was for strain St5. In conclusion, this is the first report of the isolation of autochthonous bacteria for a Loricariidae, recommending the bacterium Enterococcus faecium as probiotic in the aquaculture creation of King Tiger Pleco.

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亚马逊观赏鱼Hypancistrus sp. (Siluriformes, Loricariidae)中具有益生菌潜力细菌的筛选与分离

本研究的目的是从王虎(Hypancistrus sp.)的胃肠道中筛选出具有益生菌潜力的本土细菌，用于未来的圈养生产。乳酸菌的分离采用12份标本(8.31±0.28 cm和12.60±1.13 cm)，取标本肠，浸泡，无菌生理盐水匀浆。在MRS琼脂上从肠道中分离到21株菌株，选择5株(St1、St2、St3、St4、St5)进行体外实验。3株菌株(St2、St3、St5)每小时最大生长量最高，终浓度为109 CFU。24小时内。在NaCl(0.5 ~ 1.5%)和pH在5 ~ 6范围内的条件下，只有菌株St3和St5的生长速度最快(p<0.05)。菌株St5对胆盐的耐药性最大。综上所述，本研究首次分离出一种Loricariidae科的本土细菌，推荐将屎肠球菌作为大王虎养殖中的益生菌。

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