Photosynthetic response of citrus to Citrus tristeza virus isolates with potential for cross-protection

IF 0.7 4区农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY

Pesquisa Agropecuaria Brasileira Pub Date : 2023-01-01 DOI:10.1590/s1678-3921.pab2023.v58.03290

Rosalba Contreras-Maya, Angel Villegas-Monter, Nicacio Cruz-Huerta, Amalio Santacruz-Varela, Laura D. Ortega-Arenas, Daniel-Leobardo Ochoa-Martínez

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引用次数: 0

Abstract

Abstract The objective of this work was to evaluate the photosynthetic response of citrus varieties to the inoculation of isolates T30 and T3 of Citrus tristeza virus (CTV) characterized as having potential to provide cross-protection against severe CTV isolates in citrus. Citrus plants of 'Campbell' orange, 'Persian' lime, and 'Key' lime were subjected to inoculations by both isolates by patch. Noninoculated plants were used as the control. Symptom expression, photosynthetic and transpiration rates, and stomatal conductance were evaluated by IRGA. Stomatal density and size were evaluated by epidermal impressions. The CTV isolates were also evaluated and molecularly characterized by RT-PCR and Sanger sequencing. 'Campbell' orange and 'Persian' lime plants remained asymptomatic after the inoculations. The inoculation treatments with both CTV isolates did not reduce photosynthetic capacity, transpiration rate, and stomatal conductance, in comparison with the control. Stomatal density and size varied according to the citrus species. T30 and T3 do not affect the photosynthetic responses of 'Campbell' orange and 'Persian' lime; therefore, these isolates have the potential to provide cross-protection to citrus varieties against severe isolates of CTV.

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柑橘对具有交叉保护潜力的柑橘tristeza病毒分离株的光合反应

摘要本研究旨在评价柑橘品种对接种柑橘tristeza病毒(CTV)分离株T30和T3的光合反应，该分离株具有交叉保护柑橘严重CTV分离株的潜力。对‘Campbell’橙、‘波斯’酸橙和‘Key’酸橙柑橘植株进行了贴片接种。以未接种植株为对照。用IRGA评价症状表达、光合速率和蒸腾速率以及气孔导度。通过表皮印痕评价气孔密度和大小。通过RT-PCR和Sanger测序对CTV分离株进行了评价和分子表征。“坎贝尔”橙和“波斯”酸橙在接种后仍无症状。与对照相比，接种两种CTV菌株均未降低光合能力、蒸腾速率和气孔导度。不同种类柑橘的气孔密度和大小各不相同。T30和T3不影响‘坎贝尔’橙和‘波斯’青柠的光合反应;因此，这些分离株有可能为柑橘品种提供抗CTV严重分离株的交叉保护。

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来源期刊

Pesquisa Agropecuaria Brasileira 农林科学-农业综合

CiteScore

1.20

自引率

0.00%

发文量

审稿时长

9-18 weeks

期刊介绍： Pesquisa Agropecuária Brasileira – PAB – is issued monthly by Empresa Brasileira de Pesquisa Agropecuária – EMBRAPA, affiliated to Ministry of Agriculture, Livestock and Food Supply. PAB publishes original scientific-technological articles on Plant Physiology, Plant Pathology, Crop Science, Genetics, Soil Science, Food Technology and Animal Science. Its abbreviated title is Pesq. agropec. bras., and it should be used in bibliographies, footnotes, references and bibliographic strips.