Isolasi dan Karakterisasi Bakteri Asosiasi Daun Cymodocea serrulata di Perairan Pulau Panjang, Jepara

IF 0.5 4区 地球科学 Q3 Earth and Planetary Sciences
Alin Setiyorini, Delianis Pringgenies, Ali Ridlo
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引用次数: 0

Abstract

Lamun merupakan jenis tumbuhan berbunga yang mampu hidup terendam di laut dan memiliki banyak manfaat. Lamun memiliki banyak peran penting bagi ekossitem laut salah satu contohnya adalah sebagai sediment trap dan juga pemfiksasi CO2. Lamun juga memiliki fungsi penting lain bagi biota yang hidup di ekosistem lamun, mulai dari tempat berlindung dari predator, spawning dan feeding ground. Spesies Cymodocea sp. merupakan salah satu genus lamun yang dapat ditemukan di Pulau Panjang, Jepara. Lamun memiliki organisme asosiasi, salah satunya bakteri asosiasi yang memegang peranan penting secara biologi dan ekologis antar interaksi tumbuhan lamun dengan lingkungannya. Penelitian ini bertujuan untuk mengetahui spesies lamun yang digunakan dan mengetahui adanya bakteri asosiasi pada daun lamun tersebut. spesies lamun diidentifikasi secara morfologi sesuai buku panduan identifikasi LIPI. Bakteri asosiasi lamun diisolasi dari daun lamun dengan menggunakan metode pengenceran berseri. Isolat bakteri tersebut dikarakterisasi secara morfologi untuk mendapatkan isolat bakteri dengan ciri morfologi yang berbeda. Lamun dan bakteri asosiasi dapat menghasilkan senyawa bioaktif yang mirip antara keduanya sehingga dapat dimanfaatkan dalam bidang bioteknologi dan farmasi laut. Hasil penelitian menunjukkan bahwa spesies lamun yang digunakan adalah jenis Cymodocea serrulata. Hasil penelitian juga menunjukkan bahwa terdapat 7 isolat bakteri asosiasi daun lamun yang berhasil diisolasi dan memiliki karakteristik morfologi yang berbeda. Keberadaan bakteri asosiasi pada daun lamun ini memberikan informasi tentang terjadinya hubungan interaksi antara bakteri dan tumbuhan lamun. Seagrass is a type of flowering plant that can live submerged in the sea and has many benefits. Seagrass has many important roles for marine ecosystems, one example is as a sediment trap and also a CO2 fixer. Seagrasses also have other important functions for biota that live in seagrass ecosystems, starting from shelter from predators, spawning and feeding ground. Species Cymodocea sp. is one of the seagrass genera that can be found on Panjang Island, Jepara. Seagrass has associated organisms, one of which is associated bacteria which play an important role biologically and ecologically in the interaction between seagrass plants and their environment. This study aims to determine the species of seagrass used and to determine the presence of associated bacteria in the seagrass leaves. Seagrass species were identified morphologically according to the LIPI identification guide. Seagrass-associated bacteria were isolated from seagrass leaves using serial dilution method. The bacterial isolates were characterized morphologically to obtain bacterial isolates with different morphological characteristics. Seagrasses and associated bacteria can produce bioactive compounds that are similar between the two so that they can be used in marine biotechnology and pharmaceuticals. The results showed that the seagrass species used was Cymodocea serrulata. The results also showed that there were 7 bacterial isolates associated with seagrass leaves which had different morphological characteristics. The presence of associated bacteria on seagrass leaves provides information about the occurrence of an interaction relationship between bacteria and seagrass plants.
在杰帕拉岛的长岛海水中,细菌分离和分类
海草是一种开花植物,能够在水下生活并从中受益。海草对海洋生态系统有许多重要作用,其中一个例子是作为一个陷阱和二氧化碳稀释剂。海葵对海葵生态系统的生物也有其他重要的作用,从躲避捕食者、产卵和觅食的地方开始。一种Cymodocea sp,是在长岛Jepara发现的海葵属之一。海草有一种联合生物,一种协会细菌,它在海草与环境的相互作用之间在生物学和生态上发挥着重要作用。这项研究的目的是了解所使用的海草物种,并了解海草叶子中可能存在的细菌。海草物种根据LIPI标识手册的形态识别。海草协会的细菌通过系列稀释方法从海草叶子中分离出来。这种细菌的异构体经过形态分类,以获得具有不同形态特征的细菌异构体。海草和细菌协会可以在这两者之间产生类似的生物活性化合物,从而在生物技术和海洋制药领域发挥作用。研究表明,所使用的海葵是一种Cymodocea serrulata。研究还发现,海草叶子协会成功地分离了7种细菌,并具有不同的形态特征。海草叶子上存在的细菌提供了细菌和海草之间相互作用的信息。Seagrass是一种可以在海洋中潜水并拥有许多好处的漂浮植物。海草对海洋生态系统有很多重要的roles,其中一个解释是一个沉积的陷阱,同时也是一个二氧化碳溶液。Seagrasses还有另一个重要的功能,用于深海生态系统的生物,从躲避捕食者、spawning和觅食土地开始。在杰帕拉,在长岛可以找到的海草类物种之一。海洋草有组织,它是一种重要的生物角色,在海洋植物和环境之间相互作用。这项研究旨在确定已用海草的物种,并确定与海草叶有关的培养基。Seagrass物种被认为是根据LIPI标识指南进行的形态标记。seagrass -associated细菌通过串行方法与seagrass绝缘体分离。噬菌体被烧焦的形态学与不同的形态学特征相互排斥。Seagrasses和副细菌可以在两者之间产生类似的生物活性化合物,这样它们就可以在海洋生物技术和制药中使用。最近的一位代表表示,过去使用的海草是一种Cymodocea serlata。results还指出,有7种不同形态特征的孤立微生物。细菌相关的例子提供了关于细菌和海洋植物之间潜在关系关系的信息。
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来源期刊
Journal of Marine Research
Journal of Marine Research 地学-海洋学
自引率
0.00%
发文量
1
审稿时长
3 months
期刊介绍: The Journal of Marine Research publishes peer-reviewed research articles covering a broad array of topics in physical, biological and chemical oceanography. Articles that deal with processes, as well as those that report significant observations, are welcome. In the area of biology, studies involving coupling between ecological and physical processes are preferred over those that report systematics. Authors benefit from thorough reviews of their manuscripts, where an attempt is made to maximize clarity. The time between submission and publication is kept to a minimum; there is no page charge.
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