Detection of Acute Kidney Injury Induced by Gentamicin in a Rat Model by Aluminum-Foil-Assisted ATR-FT-IR Spectroscopy

Abstract

Fourier transform infrared (FT-IR) spectroscopy has attracted great attention in the field of disease diagnostics for many years. Yet, the clinical translation of FT-IR spectroscopy has been rather slow because of several bottlenecks. This work explores the potential of aluminum foil-assisted attenuated total reflectance-FT-IR (ATR-FT-IR) spectroscopy, which is a simple and economical FT-IR sampling technique, to detect acute kidney injury (AKI) induced by gentamicin in a rat model. It was found that partial least squares-discriminant analysis (PLS-DA) could successfully discriminate between the AKI status and the healthy status with the plasma samples of the rats. An in-depth discussion on the advantages of aluminum-foil-assisted ATR-FT-IR spectroscopy in FT-IR-based disease diagnostics is also provided.