Monitoring Cutaneous Leishmaniasis Lesions in Mice Undergoing Topical Miltefosine Treatment

IF 2.3 Q3 PHARMACOLOGY & PHARMACY
Laura Fernanda Neira, Julio Cesar Mantilla, Patricia Escobar
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Abstract

A study was conducted on BALB/c mice infected with Leishmania (Leishmania) amazonensis to analyse the effects of 0.5% miltefosine (MTF) hydrogel treatment on cutaneous leishmaniasis (CL) lesions. The mice were treated for 25 days topically, and lesion sizes, parasite loads, histopathology, ultrastructure, cytokines including interleukin 4 (IL-4), tumour necrosis factor alfa (TNFα), interferon gamma (IFNγ), IL-10, and vascular endothelial growth factor (VEGF) profiles were evaluated on days 0, 12, 25, and 85. After 12 days of treatment, the lesion sizes and parasite numbers decreased. By day 60 post treatment, there were no lesions and only a few parasites. At day 25, there was a temporary papillomatosis reaction, an increase in mast cells, a few giant cells, and granulomas, and a decrease in diffuse inflammatory infiltrate and parasites. Transmission electron microscopy (TEM) examination showed early ultrastructural changes, including macrophages without parasites and vacuoles containing electrodense material. At the different evaluated times, the cytokine regulation indexes (ICRs) decreased for IL-4, TNFα, and VEGF. According to the study, the 0.5% MTF hydrogel was effective and showed positive results from the early stages of usage. The MTF directly targeted parasites, downregulated the release of IL-4, TNFα, and VEGF, increased mast cell production, and induced granuloma reaction during evaluation periods.
局部米替福辛治疗小鼠皮肤利什曼病病变监测
本研究对感染亚马逊利什曼原虫(Leishmania)的BALB/c小鼠进行了研究,分析0.5%米替福辛(MTF)水凝胶治疗对皮肤利什曼病(CL)病变的影响。小鼠局部治疗25天,在第0、12、25和85天评估病变大小、寄生虫载量、组织病理学、超微结构、细胞因子包括白细胞介素4 (IL-4)、肿瘤坏死因子α (TNFα)、干扰素γ (IFNγ)、IL-10和血管内皮生长因子(VEGF)谱。治疗12天后,病变大小和寄生虫数量下降。到治疗后第60天,没有病变,只有少量寄生虫。第25天,出现暂时性乳头状瘤反应,肥大细胞、少量巨细胞和肉芽肿增加,弥漫性炎症浸润和寄生虫减少。透射电镜(TEM)检查显示早期超微结构改变,包括无寄生虫的巨噬细胞和含有电致密物质的液泡。在不同的评估时间,细胞因子调节指数(ICRs) IL-4、tnf - α、VEGF均下降。根据这项研究,0.5% MTF水凝胶是有效的,从使用的早期阶段就显示出积极的结果。在评估期间,MTF直接靶向寄生虫,下调IL-4、TNFα和VEGF的释放,增加肥大细胞的产生,并诱导肉芽肿反应。
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来源期刊
Scientia Pharmaceutica
Scientia Pharmaceutica Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
4.60
自引率
4.00%
发文量
67
审稿时长
10 weeks
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