Determination of Lethal Dose 50 for Induced Mutagenesis in Soybean [Glycine max (L.) Merril] cv. Gepak Kuning Through Ethyl Methane Sulfonate Mutagen

Nilahayati ., Rd. Selvy Handayani, Nazimah ., Muhammad Sani Alfikri Harahap, Gusti Irawan, Rosmaina .
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Abstract

Background: The improvement of the genetic and agronomic properties of cultivated plants can be carried out by various plant breeding methods, including mutation breeding. Mutations in nature are very few and rare. Ethyl Methane Sulfonate (EMS) is a chemical mutagen that can cause random mutations resulting in changes in genetic material. This study aims to determine the effect of EMS on the germination of soybean (cv. Gepak Kuning) seeds. Methods: The research was conducted at the Faculty of Agriculture Laboratory, Universitas Malikussaleh, Indonesia, in January 2023. The concentrations of EMS used were 0%, 0.025%, 0.05%, 0.1%, 0.25%, 0.5% and 1% with a soaking time of 4 hours. EMS concentration of 0% was used as a control treatment. The observed variables were made as percentage of germination (%) and seedling height at 7th day and 14th day after planting. Result: The results showed that an increase in EMS concentration affected the percentage of germination of soybean (cv. Gepak Kuning) sprouts. A decrease in the percentage of germination was found at an EMS concentration of 0.5%, while a decrease in seedling height had begun to be seen at an EMS concentration of 0.25%. The results of the analysis with CurveExpert 1.4 software showed that the LD50 value of the seeds of soybean (cv. Gepak Kuning) treated with EMS mutagen was at a concentration of 0.63%, the LD20 dan LD30 value was at a concentration of 0.25% and 0.41%, respectively.
大豆甘氨酸诱导诱变致死量50的测定美林的简历。格百库宁通过乙基甲烷磺酸盐诱变剂
背景:栽培植物的遗传和农艺性状的改善可以通过多种植物育种方法进行,包括突变育种。自然界的突变是非常少和罕见的。乙基甲烷磺酸盐(EMS)是一种化学诱变剂,可以引起随机突变,导致遗传物质的变化。本研究旨在确定EMS对大豆(cv。葛柏(古宁)种子。方法:研究于2023年1月在印度尼西亚马里库萨勒大学农业学院实验室进行。EMS的浓度分别为0%、0.025%、0.05%、0.1%、0.25%、0.5%和1%,浸泡时间为4小时。EMS浓度为0%作为对照处理。观察变量分别为播种后第7天和第14天的发芽率(%)和苗高。结果:EMS浓度的增加对大豆发芽率(cv。葛柏(古宁)豆芽。当EMS浓度为0.5%时,萌发率下降,而当EMS浓度为0.25%时,幼苗高度开始下降。利用CurveExpert 1.4软件分析结果表明,大豆种子的LD50值(cv。EMS诱变剂处理后,其LD20和LD30浓度分别为0.25%和0.41%。
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