Evaluation of Lacc134 Oxidoreductase of Ganoderma multistipitatum in Detoxification of Dye Wastewater under Different Nutritional Conditions

IF 2.1 Q3 MICROBIOLOGY

Microbiology Research Pub Date : 2023-09-18 DOI:10.3390/microbiolres14030095

Eman A. Alhomaidi, Aisha Umar, Salam S. Alsharari, Sami Alyahya

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Abstract

In the present study, we investigated the effects of different carbon sources (glucose, sucrose, and maltose) on laccase production from mycelium of Ganoderma multistipitatum grown on malt extract agar plates. The preliminary screening test was performed on the guaiacol plate, where a maroon brown zone formed after laccase oxidation. A few pure mycelial discs of Ganoderma species were transferred into submerged fermentation nutrient broth. The nutrient medium of submerged fermentation at 20 g of glucose revealed the highest laccase activities (2300 U/L) than other carbon sources. The interesting results also shown by inorganic NaNO3 in the production of maximum laccase (7800 ± 1.1 U/L). The organic nitrogen inducer, namely yeast extract, exhibited 5834 U/L laccase activity and a potential source of laccase secretion. The results concluded that C and N inducers enhanced the laccase production. This production process is eco-friendly and effective in the removal of dye from water. Laccase from the cultural broth was partially purified by SDS-PAGE for molecular weight determination, while Native-PAGE confirmed the laccase band after staining with guaiacol. The Km and Vmax values of Lacc134 were 1.658 mm and 2.452 mM min−1, respectively. The Lacc134 of this study effectively removed the Remazol Brilliant Blue R (RBBR) dye (extensively used in textile industries and wastewater). For dye removal capacity, 2.0 mg, 4.0 mg, 5.0 mg, and 6.0 mg were used, from which 6.0 mg was most effective in removal (85% and 88%) dye concentration in 1st and 2nd h interval treatment, respectively. Total organic carbon (TOC) quantity after dye removal percentage in the first- and second-hour time interval was 62% and 89%, respectively, at 30 g glucose. According to the experimental finding of this study, the breakdown products catalyzed by Lacc134 are less hazardous due to lower molecular weight than the dye itself.

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不同营养条件下多刺灵芝Lacc134氧化还原酶对染料废水脱毒作用的评价

在本研究中，我们研究了不同碳源(葡萄糖、蔗糖和麦芽糖)对生长在麦芽提取物琼脂板上的多刺灵芝菌丝体产漆酶的影响。在愈创木酚板上进行初步筛选试验，漆酶氧化后形成褐褐色带。将几种纯种灵芝菌丝盘转移到深层发酵营养液中。在葡萄糖含量为20 g的营养培养基中，漆酶活性最高(2300 U/L)。无机NaNO3在最大漆酶产量(7800±1.1 U/L)方面也显示出有趣的结果。有机氮诱诱剂酵母提取物的漆酶活性为5834 U/L，是漆酶分泌的潜在来源。结果表明，C和N诱导剂促进了漆酶的产生。这种生产工艺是环保和有效的去除水中的染料。用SDS-PAGE对培养液中的漆酶进行部分纯化以测定分子量，用愈创木酚染色后用Native-PAGE证实漆酶条带。Lacc134的Km和Vmax分别为1.658 mm和2.452 mm min−1。本研究的Lacc134有效地去除了Remazol Brilliant Blue R (RBBR)染料(广泛用于纺织工业和废水中)。染料去除率分别为2.0 mg、4.0 mg、5.0 mg和6.0 mg，其中6.0 mg在处理第1 h和第2 h时对染料去除率最高，分别为85%和88%。在30 g葡萄糖条件下，第1 h和第2 h脱染后总有机碳(TOC)含量分别为62%和89%。根据本研究的实验发现，Lacc134催化的分解产物由于分子量比染料本身更小，因此危险性更小。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbiology Research MICROBIOLOGY-

CiteScore

1.90

自引率

6.70%

发文量

审稿时长

10 weeks

期刊介绍： Microbiology Research is an international, online-only, open access peer-reviewed journal which publishes original research, review articles, editorials, perspectives, case reports and brief reports to benefit researchers, microbiologists, physicians, veterinarians. Microbiology Research publishes ‘Clinic’ and ‘Research’ papers divided into two different skill and proficiency levels: ‘Junior’ and ‘Professional’. The aim of this four quadrant grid is to encourage younger researchers, physicians and veterinarians to submit their results even if their studies encompass just a limited set of observations or rely on basic statistical approach, yet upholding the customary sound approach of every scientific article.