Composition of bacterial microbiome in sputum of patients with localized forms of pulmonary tuberculosis

Q4 Medicine

Pulmonologiya Pub Date : 2023-09-06 DOI:10.18093/0869-0189-2023-33-5-645-656

V. G. Druzhinin, E. D. Baranova, P. S. Demenkov, Ju. V. Zakharova, M. B. Lavryashina, A. V. Larionov, L. V. Matskova, A. V. Shabaldin, T. V. Pyanzova

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引用次数: 0

Abstract

Recent studies have shown that the respiratory bacterial microbiome has an impact on the development of pulmonary tuberculosis. Changes in the composition of the microbiome have been associated with the pathogenesis of Mycobacterium tuberculosis infection, response to therapy, and clinical outcomes of the disease. To date, the composition of the respiratory microbiome has not been studied in patients with localized forms of pulmonary tuberculosis. Methods. In the present study, the taxonomic composition of the sputum microbiome of 14 patients with localized forms of pulmonary tuberculosis (tuberculomas) and 14 healthy donors in the comparison group was analyzed by sequencing (NGS) of the V3 – V4 region of the bacterial gene encoding 16S rRNA . Results. The sputum microbiomes of the patients and the control group did not have significant differences in the species richness index (Shannon). However, the patients showed a decrease in the uniformity index, another parameter of alpha diversity. Bacterial community structures (beta diversity) did not differ significantly between patients with localized forms of tuberculosis and healthy subjects. In patients with limited forms of tuberculosis, contrary to the decrease in the content of representatives of the phyla Fusobacteria , TM7 , Tenericutes , Spirochaetes , and SR1 , and of the genera Dialister , Mycoplasma , and Filifactor in the sputum, no clear dominance of any bacterial taxon was observed. Conclusion. Certain alpha and beta diversity parameters that characterize the sputum microbiome of patients with localized forms of pulmonary tuberculosis need to be confirmed in independent large-scale studies to further understand the role of the sputum microbiota in the development of localized forms of pulmonary tuberculosis. Determination of Prevotella titers in the sputum of these patients holds promise for the diagnosis of localized forms of pulmonary tuberculosis and the search for their genomic markers.

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局限性肺结核患者痰中细菌微生物群的组成

最近的研究表明，呼吸道细菌微生物组对肺结核的发展有影响。微生物组组成的变化与结核分枝杆菌感染的发病机制、对治疗的反应和该病的临床结果有关。迄今为止，呼吸道微生物组的组成尚未在局部肺结核患者中进行研究。方法。本研究采用16S rRNA细菌基因的V3 - V4区测序(NGS)分析了14例局限性肺结核(结核瘤)患者和14例对照组健康供者的痰微生物组的分类组成。结果。患者和对照组的痰微生物群落物种丰富度指数(Shannon)无显著差异。然而，患者表现出α多样性的另一个参数均匀性指数下降。细菌群落结构(β多样性)在局部结核患者和健康受试者之间没有显著差异。在局限性结核患者中，与梭菌门、TM7、微内菌门、螺旋体门和SR1以及Dialister属、支原体属和Filifactor属的代表性细菌在痰中的含量下降相反，没有观察到任何细菌分类群的明显优势。结论。某些表征局限性肺结核患者痰菌群的α和β多样性参数需要在独立的大规模研究中得到证实，以进一步了解痰菌群在局限性肺结核发展中的作用。测定这些患者痰中的普氏菌滴度有望诊断局部形式的肺结核并寻找其基因组标记。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pulmonologiya Medicine-Pulmonary and Respiratory Medicine

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： The aim of this journal is to state a scientific position of the Russian Respiratory Society (RRS) on diagnosis and treatment of respiratory diseases based on recent evidence-based clinical trial publications and international consensuses. The most important tasks of the journal are: -improvement proficiency qualifications of respiratory specialists; -education in pulmonology; -prompt publication of original studies on diagnosis and treatment of respiratory diseases; -sharing clinical experience and information about pulmonology service organization in different regions of Russia; -information on current protocols, standards and recommendations of international respiratory societies; -discussion and consequent publication Russian consensus documents and announcement of RRS activities; -publication and comments of regulatory documents of Russian Ministry of Health; -historical review of Russian pulmonology development. The scientific concept of the journal includes publication of current evidence-based studies on respiratory medicine and their discussion with the participation of Russian and foreign experts and development of national consensus documents on respiratory medicine. Russian and foreign respiratory specialists including pneumologists, TB specialists, thoracic surgeons, allergists, clinical immunologists, pediatricians, oncologists, physiologists, and therapeutists are invited to publish article in the journal.