Step-by-step Dissection Protocol of Apicotermitinae Worker (Blattaria: Isoptera)

IF 0.7 4区农林科学 Q4 ENTOMOLOGY

Sociobiology Pub Date : 2023-10-09 DOI:10.13102/sociobiology.v70i4.8503

Rayssa Almeida-Azevedo, João Alves-Oliveira, José Wellington Morais, Elizabeth Franklin, Renato Almeida de Azevedo

引用次数: 0

Abstract

Apicotermitinae are soldierless termites highly abundant in tropical forests. The taxonomy of this subfamily is based on characters of worker cast and winged forms when present. However, the procedures necessary to dissect termite workers to observe their external and internal morphological characteristics are not well detailed in any study. Here, we describe a step-by-step protocol for worker dissection of soldierless termite species. We suggest the use of Polyvinyl Alcohol (PVA) for cleaning and visualization of the gizzard and enteric valve, and glycerin to remove tergites and sternites and describe in detail the dissection of the gizzard and enteric valve, and how visualize the insertion of Malpighian tubules.

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顶白蚁科工蜂分步解剖规程(白蚁目:等翅目)

顶白蚁是热带森林中大量存在的无蚁白蚁。这个亚科的分类是基于工人铸造和翅形式的特征，当存在。然而，解剖白蚁以观察其外部和内部形态特征所必需的程序在任何研究中都没有很详细。在这里，我们描述了一步一步的协议工人解剖无兵白蚁物种。我们建议使用聚乙烯醇(PVA)清洁和可视化砂囊和肠瓣膜，甘油去除疣体和胸骨，并详细描述砂囊和肠瓣膜的解剖，以及如何可视化马尔比氏小管的插入。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sociobiology 生物-昆虫学

CiteScore

1.60

自引率

11.10%

发文量

审稿时长

3 months

期刊介绍： SOCIOBIOLOGY publishes high quality articles that significantly contribute to the knowledge of Entomology, with emphasis on social insects. Articles previously submitted to other journals are not accepted. SOCIOBIOLOGY publishes original research papers and invited review articles on all aspects related to the biology, evolution and systematics of social and pre-social insects (Ants, Termites, Bees and Wasps). The journal is currently expanding its scope to incorporate the publication of articles dealing with other arthropods that exhibit sociality. Articles may cover a range of subjects such as ecology, ethology, morphology, population genetics, physiology, toxicology, reproduction, sociobiology, caste differentiation as well as economic impact and pest management.