Single-wavelength colorimetric tests for low-cost estimation of bacterial activity, chemical oxygen demand, and turbidity in domestic wastewater

IF 2 Q3 Environmental Science

Water Quality Research Journal of Canada Pub Date : 2023-10-09 DOI:10.2166/wqrj.2023.110

Sahashransu Satyajeet Mahapatra, Amoor S. Vishwanathan

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引用次数: 0

Abstract

Abstract Monitoring the characteristics of wastewater can enable a better choice of the options available for its treatment and also provide a rational basis for the surveillance of the spread of waterborne diseases. Our work focused on bringing down the costs associated with the estimation of the biological and physico-chemical parameters of wastewater at a common wavelength of 600 nm. We demonstrated the advantages of using the Dye Reduction-based Electron-transfer Activity Monitoring (DREAM) assay to estimate bacterial activity as an alternative to the bacterial colony count method. This assay relies on the colorimetric measurement of the extent of reduction of methylene blue in response to bacterial electron transfer. We also present the results of estimating COD and turbidity, using the standard methods of wastewater analysis, at the same wavelength of 600 nm using samples having known values of chemical oxygen demand (COD) and turbidity. Our results open up the possibility of replacing expensive spectrophotometers with LED-based, microcontroller enabled colorimeters that can be easily automated. The results of our study set the stage for the establishment of a greater number of wastewater analysis laboratories in economically backward regions which can in turn support measures to improve sanitation facilities.

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低成本估算生活废水中细菌活性、化学需氧量和浊度的单波长比色试验

监测废水的特性可以更好地选择现有的处理方案，也为监测水传播疾病的传播提供了合理的依据。我们的工作重点是降低废水在600纳米波长下的生物和物理化学参数估算的相关成本。我们证明了使用基于染料还原的电子转移活性监测(DREAM)测定来估计细菌活性作为细菌菌落计数方法的替代方法的优势。该分析依赖于亚甲基蓝响应细菌电子转移的还原程度的比色测量。我们还介绍了使用废水分析的标准方法，在600 nm的相同波长下，使用已知化学需氧量(COD)和浊度值的样品估算COD和浊度的结果。我们的研究结果开辟了用基于led的、可轻松自动化的微控制器的色度计取代昂贵的分光光度计的可能性。我们的研究结果为在经济落后地区建立更多的废水分析实验室奠定了基础，这反过来又可以支持改善卫生设施的措施。

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来源期刊

Water Quality Research Journal of Canada WATER RESOURCES-

CiteScore

1.70

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Water Quality Research Journal publishes peer-reviewed, scholarly articles on the following general subject areas: Impact of current and emerging contaminants on aquatic ecosystems Aquatic ecology (ecohydrology and ecohydraulics, invasive species, biodiversity, and aquatic species at risk) Conservation and protection of aquatic environments Responsible resource development and water quality (mining, forestry, hydropower, oil and gas) Drinking water, wastewater and stormwater treatment technologies and strategies Impacts and solutions of diffuse pollution (urban and agricultural run-off) on water quality Industrial water quality Used water: Reuse and resource recovery Groundwater quality (management, remediation, fracking, legacy contaminants) Assessment of surface and subsurface water quality Regulations, economics, strategies and policies related to water quality Social science issues in relation to water quality Water quality in remote areas Water quality in cold climates The Water Quality Research Journal is a quarterly publication. It is a forum for original research dealing with the aquatic environment, and should report new and significant findings that advance the understanding of the field. Critical review articles are especially encouraged.