The influence of the vagus nerve and indole derivative SS-68 on excitation processes in the SA node

Q3 Pharmacology, Toxicology and Pharmaceutics
Anatoliy A. Nechepurenko, Pavel A. Galenko-Yaroshevsky, Vladimir M. Pokrovskiy, Anait V. Zelenskaya, Konstantin F. Suzdalev, Svetlana A. Lebedeva, Natalia M. Makhnova, Alexandr V. Maksemyuk, Ivan A. Minenko, Roman V. Nikitin, Valeriy G. Abushkevich†
{"title":"The influence of the vagus nerve and indole derivative SS-68 on excitation processes in the SA node","authors":"Anatoliy A. Nechepurenko, Pavel A. Galenko-Yaroshevsky, Vladimir M. Pokrovskiy, Anait V. Zelenskaya, Konstantin F. Suzdalev, Svetlana A. Lebedeva, Natalia M. Makhnova, Alexandr V. Maksemyuk, Ivan A. Minenko, Roman V. Nikitin, Valeriy G. Abushkevich†","doi":"10.18413/rrpharmacology.9.10054","DOIUrl":null,"url":null,"abstract":"Introduction: Atrial fibrillation (AF) is the most common form of cardiac arrhythmias. Studying the pathogenesis of this pathological process will make it possible to look for new methods of treating AF and to predict its occurrence in a more targeted way. The aim of the study was to identify the components of the takeover process of central rhythmogenesis by the SA node in the conditions of atrial fibrillation when stimulating the vagus nerve and using substance SS-68. Materials and Methods: The experiments were conducted on 30 frogs and 90 cats. In frogs, the activity of the regions of the medulla oblongata synchronous with the heart rhythm was determined in a high-frequency electromagnetic field. In cats, proximal and distal foci of luminescence in the vagus nerve (VN) and pools of pacemaker cells (PCs) in the sinoatrial node were visualized under topical and general anesthesia, using a KELSY scanner with a microscope video capture unit while stimulating VN and using SS-68. Results and Discussion: The stimulation of VN with volleys of electrical impulses and the introduction of SS-68 increase the foci of luminescence in the nerve and unite the PC pools. This way, under general anesthesia in comparison with topical anesthesia, the area of the proximal focus of VN luminescence decreased by 83.8%, and the distal focus – by 44.9%. Against the background of general anesthesia, the area of the proximal focus of luminescence when stimulating VN with volleys of electrical impulses was by 76.0% larger than before stimulation, and the distal focus – by 72.5%. After the administration of SS-68, there was an increase in the foci of luminescence: under general anesthesia, when compared with topical anesthesia, the area of the proximal foci of luminescence decreased by 86.8%, and the distal one – by 67.1%. Under general anesthesia, the area of the proximal focus of luminescence under conditions of stimulating VN with volleys of electrical impulses was by 82.2% larger than before stimulation and the distal one – by 78.2%. When signals from the brain arrive simultaneously through VN at the PC pools, they are absorbed by the PC pools; the focus of early depolarization becomes wide, which prevents the development of AF. The increased synchronizing influence of VN may be one of the methods for treating autonomic AF, and if its influence decreases, it can be a prognostic factor for the occurrence of recurrent AF. Conclusion: The tonic effect of VN on the heart rhythm through electrical stimulation of the former and the use of SS-68 is manifested in a decreased heart rate: the difference between the initial heart rhythm and the minimal synchronization range boundary. A decrease in the heart rate under the influence of VN prevents paroxysms of AF, but does not completely eliminate the influence of ectopic foci on it.","PeriodicalId":21030,"journal":{"name":"Research Results in Pharmacology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Research Results in Pharmacology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18413/rrpharmacology.9.10054","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
引用次数: 0

Abstract

Introduction: Atrial fibrillation (AF) is the most common form of cardiac arrhythmias. Studying the pathogenesis of this pathological process will make it possible to look for new methods of treating AF and to predict its occurrence in a more targeted way. The aim of the study was to identify the components of the takeover process of central rhythmogenesis by the SA node in the conditions of atrial fibrillation when stimulating the vagus nerve and using substance SS-68. Materials and Methods: The experiments were conducted on 30 frogs and 90 cats. In frogs, the activity of the regions of the medulla oblongata synchronous with the heart rhythm was determined in a high-frequency electromagnetic field. In cats, proximal and distal foci of luminescence in the vagus nerve (VN) and pools of pacemaker cells (PCs) in the sinoatrial node were visualized under topical and general anesthesia, using a KELSY scanner with a microscope video capture unit while stimulating VN and using SS-68. Results and Discussion: The stimulation of VN with volleys of electrical impulses and the introduction of SS-68 increase the foci of luminescence in the nerve and unite the PC pools. This way, under general anesthesia in comparison with topical anesthesia, the area of the proximal focus of VN luminescence decreased by 83.8%, and the distal focus – by 44.9%. Against the background of general anesthesia, the area of the proximal focus of luminescence when stimulating VN with volleys of electrical impulses was by 76.0% larger than before stimulation, and the distal focus – by 72.5%. After the administration of SS-68, there was an increase in the foci of luminescence: under general anesthesia, when compared with topical anesthesia, the area of the proximal foci of luminescence decreased by 86.8%, and the distal one – by 67.1%. Under general anesthesia, the area of the proximal focus of luminescence under conditions of stimulating VN with volleys of electrical impulses was by 82.2% larger than before stimulation and the distal one – by 78.2%. When signals from the brain arrive simultaneously through VN at the PC pools, they are absorbed by the PC pools; the focus of early depolarization becomes wide, which prevents the development of AF. The increased synchronizing influence of VN may be one of the methods for treating autonomic AF, and if its influence decreases, it can be a prognostic factor for the occurrence of recurrent AF. Conclusion: The tonic effect of VN on the heart rhythm through electrical stimulation of the former and the use of SS-68 is manifested in a decreased heart rate: the difference between the initial heart rhythm and the minimal synchronization range boundary. A decrease in the heart rate under the influence of VN prevents paroxysms of AF, but does not completely eliminate the influence of ectopic foci on it.
迷走神经及吲哚衍生物SS-68对窦房结兴奋过程的影响
心房颤动(AF)是最常见的心律失常形式。研究这一病理过程的发病机制,将有助于寻找治疗房颤的新方法,更有针对性地预测房颤的发生。本研究的目的是确定在房颤条件下,当刺激迷走神经并使用SS-68物质时,由窦房结接管中枢心律发生过程的组成部分。材料与方法:实验对象为30只青蛙和90只猫。在青蛙身上,在高频电磁场中测定了与心律同步的延髓区域的活动。在猫的局部和全身麻醉下,使用KELSY扫描仪和显微镜视频捕捉装置,同时刺激VN并使用SS-68,观察迷走神经(VN)的近端和远端发光灶以及窦房结的起搏器细胞池。结果与讨论:电脉冲连续刺激VN和SS-68的引入增加了神经的发光焦点,并统一了PC池。由此可见,与表面麻醉相比,全麻下VN发光近端病灶面积减少83.8%,远端病灶面积减少44.9%。在全麻背景下,电脉冲连续刺激VN时,近端发光灶面积比刺激前增大76.0%,远端发光灶面积比刺激前增大72.5%。SS-68给药后,大鼠发光灶面积增加:全麻下,与表面麻醉相比,近端发光灶面积减少86.8%,远端发光灶面积减少67.1%。在全麻条件下,电脉冲连续刺激VN时,近端发光灶面积比刺激前增大82.2%,远端发光灶面积比刺激前增大78.2%。当来自大脑的信号通过VN同时到达PC池时,它们被PC池吸收;早期去极化焦点变宽,阻止房颤的发展。VN同步作用的增强可能是治疗自主型房颤的方法之一,如果其同步作用减弱,则可能是房颤复发发生的预后因素。结论:电刺激VN和使用SS-68对心律的强直作用表现为心率降低。初始心律与最小同步范围边界之差。VN影响下的心率降低可防止房颤发作,但不能完全消除异位灶对房颤的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Research Results in Pharmacology
Research Results in Pharmacology Medicine-Pharmacology (medical)
CiteScore
1.50
自引率
0.00%
发文量
32
审稿时长
12 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信