A biocompatible double-crosslinked gelatin/ sodium alginate/dopamine/quaterniazed chitosan hydrogel for wound dressings based on 3D bioprinting technology

IF 6.8 3区 医学 Q1 ENGINEERING, BIOMEDICAL
Yueqi Lu, Jie Xu, Ya Su, Huan Fang, Jiaqi Liu, Siyao Lv, Yuen Yee Cheng, Yi Nie, Wenfang Li, Bo Pan, Kedong Song
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引用次数: 5

Abstract

Severe skin injuries can cause serious problems, which could affect the patient’s normal life, if not dealt properly in a timely and effective manner. It is an urgent requirement to develop personalized wound dressings with excellent antibacterial activity and biocompatibility to match the shape of the wound to facilitate clinical application. In this study, a bioink (GAQ) based on gelatin (Gel)/sodium alginate (SA)/ quaternized chitosan (QCS) was prepared, and GAQ hydrogel dressing grafting with dopamine (GADQ) was fabricated by an extrusion three-dimensional (3D) printing technology. QCS was synthesized by modifying quaternary ammonium group on chitosan, and its structure was successfully characterized by nuclear magnetic resonance (1H NMR) and Fourier-transform infrared spectroscopy (FT-IR). Our results showed that the GADQ hydrogel dressing that was double-crosslinked by EDC/ NHS and Ca2+ had good tensile strength, considerable swelling ratio, and effective antioxidation properties. It also showed that GADQ1.5% had 93.17% and 91.06% antibacterial activity against Staphylococcus aureus and Escherichia coli, respectively. Furthermore, the relative survival ratios of fibroblast cells seeded on these hydrogels exceeded 350% after cultured for 7 days, which proved the biocompatibility of these hydrogels. Overall, this advanced 3D-printed GADQ1.5% hydrogels with effective antioxidation, excellent antibacterial activity and good biocompatibility had a considerable application potential for wound healing.
一种基于生物3D打印技术的生物相容性双交联明胶/海藻酸钠/多巴胺/季铵化壳聚糖水凝胶伤口敷料
严重的皮肤损伤会导致严重的问题,如果不及时有效地处理,可能会影响患者的正常生活。开发与创面形状相匹配的具有良好抗菌活性和生物相容性的个性化创面敷料是迫切需要的,以方便临床应用。本研究制备了基于明胶(Gel)/海藻酸钠(SA)/季铵化壳聚糖(QCS)的生物墨水(GAQ),并采用挤压三维(3D)打印技术制备了与多巴胺(GADQ)接枝的GAQ水凝胶。在壳聚糖上改性季铵基合成了QCS,并用核磁共振(1H NMR)和傅里叶变换红外光谱(FT-IR)对其结构进行了表征。结果表明,EDC/ NHS与Ca2+双交联的GADQ水凝胶敷料具有良好的抗拉强度、可观的溶胀率和有效的抗氧化性能。GADQ1.5%对金黄色葡萄球菌和大肠杆菌的抑菌活性分别为93.17%和91.06%。培养7天后,成纤维细胞的相对存活率超过350%,证明了水凝胶的生物相容性。总之,这种先进的3d打印GADQ1.5%水凝胶具有有效的抗氧化、优异的抗菌活性和良好的生物相容性,在伤口愈合方面具有相当大的应用潜力。
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来源期刊
CiteScore
6.90
自引率
4.80%
发文量
81
期刊介绍: The International Journal of Bioprinting is a globally recognized publication that focuses on the advancements, scientific discoveries, and practical implementations of Bioprinting. Bioprinting, in simple terms, involves the utilization of 3D printing technology and materials that contain living cells or biological components to fabricate tissues or other biotechnological products. Our journal encompasses interdisciplinary research that spans across technology, science, and clinical applications within the expansive realm of Bioprinting.
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