Effect of dietary omega-3 polyunsaturated fatty acids supplementation of Astyanax lacustris males on semen quality

IF 2 4区生物学 Q1 ZOOLOGY

Neotropical Ichthyology Pub Date : 2023-01-01 DOI:10.1590/1982-0224-2023-0077

Laicia Carneiro-Leite, Lorena Pacheco da Silva, Hellen Buzollo Pazzini, Stella Indira Rocha Lobato, Laís Pedroso Borges, Yasmin dos Santos Reis, Luciane Gomes-Silva, Cristiéle da Silva Ribeiro, Rosicleire Veríssimo-Silveira, Alexandre Ninhaus-Silveira

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Abstract

Abstract This study aimed to verify whether the inclusion of omega-3 polyunsaturated fatty acids (PUFAs-ô3) in the diet of Astyanax lacustris males improves seminal quality. Four hundred fish were arranged in 20 boxes of 180 L in the recirculation system. These were fed with isoprotein diets (32% CP), until apparent satiation, with four levels of marine fish oil rich in PUFAs-ô3 inclusion (In% = 0, 3, 6, and 9), for 105 days. After, males were hormonally induced to spermiation, and semen was collected after 226 ATUs. Parameters evaluated were osmolality, seminal volume and color, spermatic concentration, sperm morphology, membrane integrity and, sperm motility (total and progressive motility, velocity curvilinear, straight line and average path, linearity and rectilinearity coefficients, trajectory oscillation, head lateral displacement amplitude, and cross-beat frequency). Seminal volume was greater in the In-0 and In-3 groups. Inclusion of PUFAs-ô3 positively influenced the kinetic parameters, as treatment with 6% and 9% of inclusion fish oil resulted in higher values for most of these parameters and did not differ statistically from each other. Thus, it is concluded that the addition of PUFAs-ô3 to the feed of breeders significantly improved the seminal quality of A. lacustris males.

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日粮中添加omega-3多不饱和脂肪酸对湖鲈雄性精液质量的影响

摘要本研究旨在验证在湖Astyanax (Astyanax lakstris)雄性饲料中添加omega-3多不饱和脂肪酸(PUFAs-ô3)是否能改善其精液质量。400条鱼被安排在20箱180升的循环系统中。这些小鼠被喂食等蛋白饲料(32% CP)，直到明显饱腹，含有四种富含PUFAs-ô3的海洋鱼油(in % = 0、3、6和9)，持续105天。然后用激素诱导雄性精子，226 ATUs后采集精液。评估的参数包括渗透压、精液体积和颜色、精子浓度、精子形态、膜完整性和精子运动(总运动和渐进运动、速度曲线、直线和平均路径、线性和直线系数、轨迹振荡、头部侧向位移幅度和交叉跳动频率)。in -0和in -3组的精液量更大。PUFAs-ô3的加入对动力学参数有积极影响，因为添加6%和9%的鱼油会导致大多数这些参数的值更高，并且彼此之间没有统计学差异。由此可见，在繁殖者的饲料中添加PUFAs-ô3可显著提高湖鳅雄性的精液质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neotropical Ichthyology 生物-动物学

CiteScore

2.80

自引率

17.60%

发文量

审稿时长

6-12 weeks

期刊介绍： Neotropical Ichthyology is the official journal of the Sociedade Brasileira de Ictiologia (SBI). It is an international peer-reviewed Open Access periodical that publishes original articles and reviews exclusively on Neotropical freshwater and marine fishes and constitutes an International Forum to disclose and discuss results of original research on the diversity of marine, estuarine and freshwater Neotropical fishes. -Frequency: Four issues per year published only online since 2020, using the ‘rolling pass’ system, which posts articles online immediately as soon as they are ready for publication. A searchable and citable Digital Object Identifier (DOI) is assigned to each article immediately after online publication, with no need to await the issue’s closing. -Areas of interest: Biology, Biochemistry and Physiology, Ecology, Ethology, Genetics and Molecular Biology, Systematics. -Peer review process: The Editor-in-Chief screens each manuscript submitted to Neotropical Ichthyology to verify whether it is within the journal’s scope and policy, presents original research and follows the journal’s guidelines. After passing through the initial screening, articles are assigned to a Section Editor, who then assigns an Associate Editor to start the single blind review process.