Electrochemical and colorimetric dual-signal detection of Staphylococcus aureus enterotoxin B based on AuPt bimetallic nanoparticles loaded Fe-N-C single atom nanocomposite

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness Pub Date : 2024-05-27 DOI:10.26599/FSHW.2022.9250168

Huan Liang , Hongcheng Liu , Haojian Lin , Guobao Ning , Xiaokang Lu , Siying Ma , Fei Liu , Hui Zhao , Canpeng Li

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Abstract

Sensitive detection of Staphylococcus aureus enterotoxin B (SEB) is of importance for preventing food poisoning from threatening human health. In this work, an electrochemical and colorimetric dual-signal detection assay of SEB was developed. The probe (Ab2/AuPt@Fe-N-C) was bound to SEB captured by Ab1, where the Ab2/AuPt@Fe-N-C triggered methylene blue degradation and resulted in the decrease of electrochemical signal. Furthermore, the probe catalyzed the oxidation of 3,3’,5,5’-tetramethyl biphenyl to generate a colorimetric absorbance at 652 nm. Once the target was captured and formed a sandwich-like complex, the color changed from colorless to blue. SEB detection by colorimetric and electrochemical methods showed a linear relationship in the concentration ranges of 0.000 2–10.000 0 and 0.000 5–10.000 0 ng/mL, with limits of detection of 0.066 7 and 0.167 0 pg/mL, respectively. The dual-signal biosensor was successfully used to detect SEB in milk and water samples, which has great potential in toxin detection in food and the environment.

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基于 AuPt 双金属纳米粒子负载 Fe-N-C 单原子纳米复合材料的金黄色葡萄球菌肠毒素 B 电化学和比色双信号检测技术

灵敏检测金黄色葡萄球菌肠毒素 B（SEB）对防止食物中毒威胁人类健康具有重要意义。本研究开发了一种 SEB 的电化学和比色法双信号检测方法。探针（Ab2/AuPt@Fe-N-C）与被 Ab1 捕获的 SEB 结合，Ab2/AuPt@Fe-N-C 触发亚甲基蓝降解，导致电化学信号下降。此外，探针还催化了 3,3',5,5'-四甲基联苯的氧化，在 652 纳米波长处产生比色吸光度。一旦目标物被捕获并形成类似三明治的复合物，颜色就会从无色变为蓝色。在 0.000 2-10.000 0 和 0.000 5-10.000 0 纳克/毫升的浓度范围内，比色法和电化学法检测 SEB 呈线性关系，检测限分别为 0.066 7 和 0.167 0 皮克/毫升。该双信号生物传感器成功用于检测牛奶和水样中的 SEB，在食品和环境毒素检测方面具有巨大潜力。

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来源期刊

Food Science and Human Wellness Agricultural and Biological Sciences-Food Science

CiteScore

8.30

自引率

5.70%

发文量

审稿时长

28 days

期刊介绍： Food Science and Human Wellness is an international peer-reviewed journal that provides a forum for the dissemination of the latest scientific results in food science, nutriology, immunology and cross-field research. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. By their effort, it has been developed to promote the public awareness on diet, advocate healthy diet, reduce the harm caused by unreasonable dietary habit, and directs healthy food development for food industrial producers.