The Effectiveness of Wild Honey In Inhibiting The Growth of Bacterium on The Positive Widal Blood Culture of Enterobacteriaceae Familia

Abstract

Honey is a product produced by bees which is believed has many benefits in the medical field. Wild honeys are more natural than livestock honey, and it has high antimicroba activities, but the effect of the antimicroba towards the bacteria on the positive widal blood culture of Enterobacteriaceae familia member was not clear yet. This research was to find out The effectiveness of wild honey in inhibiting the growth of bacteria on the positive widal blood culture of Enterobacteriaceae familia member. Method in this research was an experimental research which was using combination of diffusion and draw well method using Klebsiella pneumonia, Esherichia coli, Salmonella typhi, Serratia marcescens, and Enterobacter cloacae bacteria sample. Wild honey as a test solution with a 25%, 40%, 55%, 70%, 85% and 100% concentrate. The result is that wild honey is effective to inhibit the growth of the Enterobacteriaceae bacteria familia, the inhibition zone which is performed on the 70% Nutrien Agar Plate (NAP) concentrate medium is a minimal concentrate that sensitive to all bacteria with the  inhibition zone similar to Kloramfinekol antibiotics. S. typhi is sensitive to all wild honey concentrate within 26,5mm, 29mm, 31,5mm, 32mm, 35mm, and 38mm inhibition zone. Wild honey on E.coli and Serratia marescens is sensitive in all concentrate except on the 25% concentrate. On the Klebsiella pneumonia the wild honey is sensitive on the 70%, 85%, and 100% concentrate while on the Enterobacter cloacae the wild honey is sensitive on 55%, 70%, 85% and 100% concentrate. Conclusion : wild honey is effective to inhibit bacteria growth on the positive widal blood culture of Enterobacteriaceae familia member and there are effect of various wild honey concentrate with the growth of Enterobacteriaceae bacteria which the higher concentrate of the wild honey, the greater inhibition potency towards the bacteria.