[Detection of antibodies against Toxoplasma gondii in human sera in immunoblot procedures].

Abstract

Proteins of Toxoplasma lysate were separated by SDS-polyacrylamide gel electrophoresis with subsequent transfer to a nitrocellulose sheet by electrophoretic blotting. Patient human sera reacted predominant with protein fractions of Toxoplasma antigen of 94 kD and 35 kD. Human sera were tested by Toxoplasma-IgG ELISA and Immunofluorescence assay (IgG-IFAT, IgM-IFAT).