A Study on Basic Data on Skin Aging of Green Coffea Arabica Beans (in Kenya Nyeri)

Abstract

Physiological activity of Green Coffea Arabica beans (Nieri, Kenya) hot water and 70% ethanol extract before (GBKW, GBKE) and after 25 kGy (GBKW-r, GBKE-r) irradiation was verified to confirm the possibility as a natural material. As a results of measuring the total polyphenol content, GBKW and GBKE extract confirmed the content of 297.05 ± 0.99 mg/100 g and 671.34 ± 0.75 mg/100 g. And GBKW-r and GBKE-r extract confirmed the content of 423.47 ± 0.42 mg/100 g and 415.33 ± 0.79 mg/100 g. As a result of measuring the electron donating activity, it was confirmed that at a concentration of 500 μg/ml, GBKW and GBKE were 85.73% and 89.39%, GBKW-r and GBKE-r showed 48.31% and 68.42%. In addition, the results of ABTS radical scavenging activity, it was confirmed that at a concentration of 500 μg/ml, GBKW and GBKE were 52.66% and 67%, GBKW-r and GBKE-r showed 99.17% and 99.96%, respectively. So, all extracts GBKW, GBKE, GBKW-r and GBKE-r were found to have antioxidant activity. Subsequently, as a result of measuring cell viability through MTT assay using RAW 264.7, which is a macrophage, GBKW-r and GBKE-r showed a cell viability more than 95% at a concentration of 10 μg/ml. Afterwards, NO production inhibitory activity was conducted at a concentration of 10 μg/ml or less. As a result, it was confirmed that GBKW-r and GBKE-r showed anti-inflammatory effects of 10.99% and 35.66%, respectively, at a concentration of 10 μg/ml. Therefore, irradiatied Green Coffea Arabica Beans (in Kenya Nyeri) hot water and 70% ethanol extract have antioxidant and anti-inflammatory activity, and it is considered that there is a possibility of application as a natural material.