Panax Notoginseng Saponins Induces Apoptosis in Breast Cancer by Regulating PI3k/AKT Pathway and Suppressing Bcl-2 by Using Computer Science

Abstract

Hypothesis: I postulate that the major function of PNS in breast cancer is to induce the apoptosis and inhibit the proliferation through activate the inhibition of P13k/AKT pathway to suppress the effect of Bcl-2 and increase the Bax and caspase-3. Purpose: Breast cancer is malignant disease for human beings. The traditional therapy is mainly resection operation. Though its effect of radical cure is high, it easily metastasizes and invades the tumor. Previous studies have shown that Panax notoginseng Saponins (PNS) has inhibitory proliferation effect and relevant function on apoptosis via PI3k/AKT/mTOR pathway. The study will investigate how PNS effects apoptosis by regulating PI3k/AKT in in vitro condition. Materials and method: The cell line which will be used is 4T1-Leu breast cancer cells. The cell proliferation will be assessed by Cell Counting Kit-8, whereas the apoptotic rate will be investigated by flow cytometry and Annexin V. The relevant proteins about PI3k/AKT/mTOR pathway and apoptosis will be assessed by western blotting. Possible results: There are five possible results in cell proliferation, five results in apoptosis and 6 results in western blot in this study, it can be aggregated to 4 results. 1.PNS can inhibit proliferation and induce apoptosis of 4T1-luc breast cancer cells by suppressing the PI3K/AKT pathway; 2. PNS activates proliferation and inhibit apoptosis of 4T1-luc breast cancer cells by suppressing the PI3K/AKT/mTOR pathway. 3. PNS activates proliferation and inhibit apoptosis of 4T1-luc breast cancer cells by promoting mTOR pathway via PI3K/AKT. 4. PNS inhibit apoptosis of 4T1-luc breast cancer cells by suppressing mTOR pathway via PI3K/AKT. Conclusion: The results of the study will provide a new way to investigate the PNS in breast cancer therapy, in both apoptosis and cell signal pathway ways.