Lawsonia inermis And Hibiscus sabdariffa : Posible Histological Stains

Abstract

The ability of various concentrations of aqueous extracts of Lawsonia inermis and Hibiscus sabdariffa to stain histological tissues was demonstrated. The results with sections of tongue and kidney of the laboratory rat, cut at 6microns thickness showed that only the cellular cytoplasm was stained. However, combinations of the extracts of L. inermis with haematoxylin and H. sabdariffa with eosin gave good tissue staining with high contrast and cellular clarity. The result was compared with the usual staining with haematoxylin and eosin (H & E). It is concluded that the extracts of L. inermis and H. sabdariffa can be used as substitutes for eosin and haematoxylin respectively in routine histological preparations. Keywords : Lawsonia inermis, hibiscus sabdariffa , water extract, tissues. Tropical Veterinarian Vol. 24 (1 & 2) 2006: pp. 1-5