Comparison of Different Extraction Methods for the Genome of Enterocytozoon hepatopenaei, EHP

Abstract

In order to explore the most suitable extraction method of the Enterocytozoon hepatopenaei (EHP) genome, five methods were used to extract genomes of equal amounts of EHP, including traditional and improved phenol/chloroform extraction, enzymatic hydrolysis of chitin, marine animals DNA kit and plant genomic DNA kit. Results showed that the effect on the genomic concentration was in descending order of marine animals DNA kit (18.825 ng/µL), enzymatic hydrolysis of chitin (17.17 ng/µL), improved phenol/chloroform extraction (15.06 ng/µL), traditional phenol/chloroform extraction (10.8 ng/µL), plant genomic DNA kit (10.8 ng/µL); the effect on the genome integrity was in descending order of marine animals DNA kit, improved phenol/chloroform extraction, plant genomic DNA kit, traditional phenol/chloroform extraction, enzymatic hydrolysis of chitin. Due to the highest extraction concentration and the best integrity, this study confirms that the method of marine animals DNA kit was the most suitable extraction method for EHP genome.