Co transformation of Gus and npt II genes using Agrobacterium tumefaciens strain GV2260:p35 GUS INT in tobacco variety Samsun Maden 2421

Abstract

Co-transformation in Agrobacterium-mediated is known as an important problem. Therefore, this study aimed to co-transform the Samsun Maden variety of tobacco. For this purpose, fresh seeds of cv. Samsun Maden 2421varitey of tobacco was procured from the Bafra county open market in Samsun province, Türkiye. In this study, a well-known transformation through A. tumefaciens was used because plants for example tobacco are recalcitrant or difficult to regenerate and fail to respond to other transformation techniques. The result showed successful co-transformation of square-shaped leaf explants on all cultures, excluding 50 mg/l Kanamycin used as a selection pressure. This concentration showed minör escapes to the extent of 1.67%. Indicating that optimizing selection pressure is very important to avoid escapes in co-transformation. Moreover, the regeneration efficiency of explants is reduced considerably with each increase in selection pressure.