PHYSICAL PROPERTIES OF SOME DIHAPLOID VARIETIES AND LINES FROM THE TOBACCO TYPE PRILEP

Abstract

Oriental tobaccos in the Republic of Macedonia are selected according to classical methods, which is quite a long process. Today, one of the most commonly used biotechnological methods in plants is the doubled haploid method, whose main purpose is to shorten selection processes in field conditions, while obtaining homozygous lines in the first generation. Tobacco is an ideal plant for the production of haploid plants, tobacco crops produce an explosion of haploids that are being used today in the process of tobacco hybridization. Dihaploidization was performed by direct and rogenesis of haploid tobacco plants obtained in the laboratory in vitro at the Scientific Tobacco Institute - Prilep, using the tissue culture method. Four dihaploid lines of tobacco were included in the research, as follows: P 146-7 / 1 DH, Jk.l. 301/23DH, Hybrid 301 / n DH, Jk.l.75-301 DH and their analogues (P 146-7 / 1 Ø, Hybrid 301/23 Ø, Hybrid 301 / n Øand Jk.l.75-301 Ø).Materiality as an expression of the quality of the leaf tissue varies from 25.96 g/m² (Hybrid 301 /n DH) to 36.91 g/m² (Jk.l. 301/23 Ø).The leaf thickness of the tested dihaploid lines and tobacco varieties is within the range from 76.00 µm (Hybrid 301 / n DH) to 134.50 µm (Hybrid. 301/23 Ø).