ANTIBACTERIAL ACTIVITIES OF POLAR FRACTION OF PAPAYA LEAF ETHANOLIC EXTRACT (Carica papaya Linn.) AGAINST Escherichia coli AND Staphylococcus aureus

Abstract

Carica papaya is widely used as a traditional medicine society. Papaya leaves contain saponins, alkaloids, terpenoids, flavonoids, and tannins which are believed to be antibacterial compounds. Objective to determine the antibacterial activities polar fraction of papaya leaf ethanol extract (Carica papaya  Linn.) against  Escherichia coli and Staphylococcus aureus. Simplicia was extracted by ethanol 70% with the maceration method. Ethanolic extract was fractionated with ethanol water. Kirby Bauer diffusion method agar was used for antibacterial activities. The variable of this study was the concentration of the ethanol-water fraction of papaya leaf ethanolic extract (Carica papaya  Linn.) 10%, 15%, 20%, 25%, 30%, 100% and the growth inhibition zone of Escherichia coli and Staphylococcus aureus. The data obtained were analyzed using the One Way ANOVA statistical method. Data analysis using the One Way ANOVA statistical test showed a significant difference in the treatment group to the zone of inhibition of Staphylococcus aureus and Escherichia coli bacteria (sig=0.000). The zone of inhibition of the ethanol-water fraction against Staphylococcus aureus was the most optimum at a concentration of 100% at 14.75 mm, while the most optimum for Escherichia coli was at a concentration of 100% at 11.53 mm, but did not effective than positive control Chloramphenicol. Conclusion the ethanol-water fraction of papaya leaf ethanol extract (Carica papaya L.) could inhibit the growth of Staphylococcus aureus and Escherichia coli bacteria.