Anthelmintic effects of four medicinal plants using in vitro L3 larva stage

Abstract

In vitro anthelminthic activity of Vernonia amygdalina, Ocimum grattissimum, Nicotiana tabacum and Talinum triangulare using L3 stage larva was investigated. Standard procedure was followed for larva culturing, preparation of extract and for in vitro assay. The concentration of Vernonia amygdalina was effective at 1.0 mg/ml at 1 and 2 hours, Ocimum grattissimum at 0.5 mg/ml at 1 hour, Nicotiana tabacum at 0.125 mg/ml at 1 hour and Talinum triangulare at 0.125 mg/ml at 2 hours. The result showed that there was anthelminthic activity in the four plants after 3 hours of the experiment. Nicotiana tabacum showed a faster anthelmintic effect at a lower concentration of 0.125 mg/l and at a lower hour (1 hour) while Talinum triangulare showed a slower anthelmintic effect compared to other medicinal plants at 2 hours. The health status of ruminants can be improved through the consumption of these four medicinal plants.