Isozymes, protein and ODAP variability of grasspea (Lathyrus sativus L.) in Ethiopia

Sinet, Ethiopian Journal of Science Pub Date : 2005-10-18 DOI:10.4314/SINET.V27I2.18246

W. Tadesse, E. Bekele

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引用次数: 3

Abstract

Variability of grass pea landrace populations for isozymes, Oxalyl Diamino Propanoic acid ODAP) and protein contents were investigated following standard procedures to detect genetic variation. The result indicated that phenotypic polymorphism was observed for the three enzymes, the highest being for esterase. The degree of differentiation (FST) of the individual loci ranged from 0.031 (for ACP-3) to 0.784 (for AAT-2). The highest polymorphism was detected in populations collected from Gondar region indicating the need for priority germplasm collection and site selection for in situ conservation in this region. The ODAP and protein analyses also revealed presence of variability among the populations. Fortunately, four entries with low ODAP contents ranging from 0.14% to 0.18% were identified in populations collected from different regions indicating the variation of this trait within population and between regions. These lines with low ODAP content are obviously considered important for further breeding program. Key words/phrases : Ethiopia, grass pea , isozyme, Lathyrus sativus , ODAP ET: Ethiopian Journal of Science Vol. 27 (2) 2004: 153–160

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埃塞俄比亚草种(Lathyrus sativus L.)同工酶、蛋白质和ODAP变异

采用标准的遗传变异检测方法，研究了草豌豆地方群体同工酶、草酰二氨基丙酸(ODAP)和蛋白质含量的变异。结果表明，3种酶均存在表型多态性，其中酯酶多态性最高。各基因座的分化程度(FST)从0.031 (ACP-3)到0.784 (AAT-2)不等。在贡达尔地区收集的种群中，多态性最高，表明该地区需要优先进行种质收集和原位保护的选址。ODAP和蛋白质分析也揭示了人群之间存在变异性。幸运的是，在不同地区收集的群体中鉴定出4个ODAP含量较低的条目，范围在0.14%至0.18%之间，表明该性状在群体内和区域间存在差异。这些低ODAP含量的品系显然对进一步的育种计划具有重要意义。关键词/短语:埃塞俄比亚，草豆，同工酶，Lathyrus sativus, ODAP ET:埃塞俄比亚科学杂志27 (2)2004:153-160

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Sinet, Ethiopian Journal of Science

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