Detection of Virulence Genes in Some of Bacillus sp Isolated from Camel Urine Using PCR Analysis

Abstract

Bacillus is a genus that contains both environmental and pathogenic species that can cause food poisoning in humans as a result of consuming foods containing toxins or bacteria. In this study, the 16S rRNA gene of the bacteria was sequenced to identify bacteria that were isolated from camel urine. Detected the Bacillus enterotoxins that encoding by three virulence genes; nonhemolytic enterotoxin (NHE) gene, cytotoxin K (CytK) gene and hemolytic enterotoxin (HBL) gene hblD. Bacillus sp isolates were examined for detection Bacillus enterotoxins (NheA,hblD, and CytK ) by PCR. NheA gene was detected in two of 78 examined samples 2.5% while, cytK gene was detected in 1.2 % of the examined samples. On the other hand, hblD gene failed to be detected.