Characterization of Microsatellite Loci for the Alligator Snapping Turtle and Cross-Amplification in Two Chelydrid Species

Luke Pearson, B. Kreiser, C. Qualls
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引用次数: 1

Abstract

Abstract. The alligator snapping turtle (Macrochelys temminckii) has been proposed as a Threatened species under the US Endangered Species Act and is considered Vulnerable by the International Union for the Conservation of Nature. To provide additional markers for conservation genetic studies, we identified 60 potential microsatellite loci, with 38 being polymorphic in M. temminckii, as well as successful cross-amplification of 12 and 32 polymorphic loci in the Suwannee alligator snapping turtle (M. suwanniensis) and Eastern snapping turtle (Chelydra serpentina), respectively. These loci will be useful in genetic studies associated with informing future management decisions for M. temminckii and the other species of Chelydridae.
短吻鳄鳄龟微卫星位点的鉴定及两种Chelydrid的交叉扩增
摘要短吻鳄鳄龟(Macrochelys temminckii)已被美国《濒危物种法案》列为濒危物种,并被国际自然保护联盟视为易危物种。为了为保护遗传研究提供额外的标记,我们鉴定了60个潜在的微卫星位点,其中38个在M. temminckii中存在多态性,并分别在Suwannee短吻鳄鳄龟(M. suwanniensis)和东方鳄龟(Chelydra serpentina)中成功交叉扩增了12个和32个多态性位点。这些基因座将在遗传研究中提供有用的信息,为未来对M. temminckii和其他Chelydridae物种的管理决策提供信息。
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