The effects of earthworm powder on viability of Entamoeba histolytica (trophozoites) in vitro

Abstract

This study was accomplished in vitro to measure the viability of trophozoite by using Methylthiazol Tetrazolium (MTT) assay after the parasite was saved in vitro by using Locke-egg medium (LEM), when the parasite was treated, different concentrations of the earthworm powder were used (50,100,150,200 mg/ml) in different times (24, 48, 72 hours). Results showed the viability of trophozoite in (50 mg/ml) after (24, 48, 72h) was (74 %, 78 %, 70 %) respectively compared with control (100 %99 %95%) respectively, also in (100 mg/ml) after (24, 48, 72h) was (60 %,43 %,49 %) respectively, in (150 mg/ml) after (24, 48, 72h) was (40 %,31 %,35 %) respectively, in (200 mg/ml) after (24, 48, 72h) was (26 %,20 %,30 %) respectively compared with control while in metronidazole (24, 48, 72h) was (5 %,4%, 3%) respectively compared with the control.