Hydrodistillation of Nigella Sativa Seed and Analysis of Thymoquinone with HPLC and GC-MS

Ü. Erdoğan, M. Yılmazer, S. Erbaş
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引用次数: 7

Abstract

N. sativa seeds, commonly known as black seeds, are for headaches in traditional medicine by many Asian, Middle Eastern and Far Eastern countries. It is used to treat cough, abdominal pain, diarrhea, asthma, rheumatism and other diseases. The seeds contain both fixed and essential oils, proteins, alkaloids and saponin. Much of the biological activity of the seeds has been shown to be due to thymoquinone, the major component of the essential oil, but which is also present in the fixed oil. The essential oil of black cumin seeds, Nigella sativa L., was tested for a possible antioxidant activity. In this study, we measured the amount of thymoquinone compound in distilled water taken from aromatic water. The proportion of thymoquinone passing into the aromatic water show that it is necessary to consume the correct amount of water and essential oil, which is significantly measurable by GC MS and HPLC. Thymoquinone was dedected 790 ppm in aromatic water and 45.78% in essential oil. Sufficient analytical work was performed with this method and the results were shown.
黑草种子的加氢蒸馏及百里醌的HPLC和GC-MS分析
在亚洲、中东和远东许多国家的传统医学中,亚麻籽通常被称为黑籽,用于治疗头痛。用于治疗咳嗽、腹痛、腹泻、哮喘、风湿病等疾病。种子含有固定油和精油、蛋白质、生物碱和皂素。许多种子的生物活性已被证明是由于百里醌,精油的主要成分,但也存在于固定油。对黑孜然种子Nigella sativa L.的精油进行了可能的抗氧化活性测试。在本研究中,我们测定了从芳香水中提取的蒸馏水中百里醌化合物的含量。百里醌进入芳香水的比例表明,需要消耗适量的水和精油,GC - MS和HPLC可显著测量。百里醌在芳香水中的检出率为790 ppm,在精油中检出率为45.78%。用这种方法进行了充分的分析工作,结果得到了证明。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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