Effect of Asparagus Polysaccharides on Migration Time of Erythrocytes in Tumor-Bearing Organisms Using High Performance Capillary Electrophoresis

Abstract

This study analyzed the effect of Asparagus polysaccharides on the migration time of erythrocytes in tumor- bearing organisms. A mouse tumor model was developed in which mice were intraperitoneally injected with high, medium, and low dosages of Asparagus polysaccharides for 7 d. The erythrocytes were collected and prepared into suspensions, and the migration time of cells were measured using high performance capillary electrophoresis (HPCE). Experimental conditions included the following: capillaries, 75 mum x 50 cm; buffer for electrophoresis, phosphate solution containing hydroxypropylmethyl cellulose (0.1 mol/L, pH 7.4); injection pressure, 3.448 kPa; injection time, 10 s; separation voltage, 20 kV; column temperature, 25degC. It was found that Asparagus polysaccharides can decrease the migration time of erythrocytes in tumor-bearing organisms, which was 17.20 min for the control group, 14.33 min for the low-dosage group, 11.95 min for the medium-dosage group, 13.03 min for the high-dosage group, and 11.42 min for the normal group. Compared with the negative control group, all three dosage groups were significantly different (P < 0.01). It was also found that Asparagus polysaccharides can change the migration time of erythrocytes in tumor-bearing organisms with the tendency to the condition in normal organisms, which is possibly because Asparagus polysaccharides can change the charges density on erythrocytes surface.