Curcumin Effect on Nitropropane Alterations on the IL-1β, Tnfα & IL 6 in Submandibular Gland of Mice

Abstract

Objectives : 2-Nitropropane (2-NP) is a rat liver carcinogen. Curcumin has shown some therapeutic activities. This study aimed to investigate if curcumin has a synergistic effect on the 2-NP alterations on the IL-1β, IL 6 & TNFα in submandibular gland of Mice. Methods and materials: by intraperitoneal injection of 2-NP (100mg/kg body weight dissolved in canola oil) 2 time/week for 4 weeks. At the same time of 2-NP injection, mice of group III were orally drinking water supplemented with curcumin 50(Mg/Ml)/4weeks. Submandibular gland tissues were histologically studied. Results : the gene expression analysis showed a statistically significant upregulation of TNF-α, IL-1β, and IL-6 in group II. While, group III showed significantly downregulated expression. The apparent histopathological changes manifested in group II was less in group III. Conclusions : Curcumin may modulate the alterations of 2-NP by down-regulating proinflammatory cytokines IL-1β, IL6 and TNF α in BALB/c mice.