[Studies on immunobiological activities of periodontopathic bacteria. Comparison of the activities of soluble components from Bacteroides gingivalis and Actinomyces viscosus].

Abstract

It has been proposed that Bacteroides gingivalis and Actinomyces viscosus are most important agents to pathogenesis of the periodontitis and gingivitis. In this study, the influences of sonic extracts prepared from B. gingivalis and A. viscosus for DNA synthesis of murine lymphocytes, production of prostaglandin E2 (PGE2), collagenase and interleukin-1 (IL-1) from human peripheral monocyte were investigated. Furthermore, PGE2 and collagenase production by fibroblasts from human periodontal ligament (HPLF) and gingiva (Gin 1) stimulated with macrophage conditioned medium (MCM) cultured with bacterial sonic extracts were examined. The results obtained were as follows. 1. The sonic extracts (10 micrograms/ml protein) from B. gingivalis and A. viscosus showd low mitogenic activity to spleen cells, however, induced polyclonal B cell activation. 2. Although, the effect of these sonic extracts on the production of PGE2 and collagenase by human peripheral monocyte was not found, the induction of IL-1 production was recognized. 3. The culture supernatants of C3H/HeN mouse peritoneal macrophage stimulated with sonic extracts of B. gingivalis and A. viscosus were induced PGE2 and collagenase production by HPLF and Gin 1. These results suggest that the cellular components of B. gingivalis and A. viscosus may play the pathogenic roles in periodontal tissue destruction through the stimulation of macrophage and/or lymphocyte.