Effect of the Elicitor Hyaluronic acid on Multiplication and Indirect Regeneration of Curcuma longa L. Plant

Abstract

The aim of this study was to evaluate the effect of hyaluronic acid (HA) concentrations for multiplicationand indirect regeneration of Curcuma longa L. plant. Multiplied shoots of C. longa L. were used as explant materialsand callus for indirect regeneration. The explants cultured on Murashige and Skoog (MS) medium supplemented withdifferent concentrations of HA as0.05, 0.5, 1.5, 3.0 and 15 mg l-1 for multiplication and 0.0, 0.16, 0.33, 0.66, 1.00, 2.00and 5.00 mg l−1 for indirect regeneration. Concerning to multiplication, the highest number of shoot (3.77 shootsexplant-1) was obtained from 3 mg l−1 HA. While, the least concentrations of HA (0.16, 0.33 and 0.66 mg l-1) enhancedshoot regeneration. On the other hand, the highest concentrations of HA produced higher number of roots and heaviestweight of callus under light condition.