Detection of the Neurotoxic Amino Acid β-N-methylamino-L-alanine in Axenic Cultures of Cyanobacterial Isolates by the Application of Underivatized Method of Analysis

Abstract

There is a growing demand to establish reliable method for the detection of cyanobacterial toxins in order to bewell informed on the prevalence of cyanotoxins in our environments. This study employed the underivatizedmethod of analysis to detect the production of BMAA by the axenic cultures of cyanobacterial strains SynechocystisNPLB 2 and Nostoc MAC PCC 8009 using liquid chromatography-electrospray ionization - ion trap massspectrometry (LC/ESI-ITMS). Cyanobacterial cultures were grown in the laboratory for 12 weeks after whichBMAA was extracted using methanol and analysed by LC/ESI-ITMS. Based on retention time, mass-to-chargeratio, and ratio of the product ions, the underivatized method of analysis employed in this study showed that bothSynechocystis NPLB 2 and Nostoc MAC PCC 8009 strains produced BMAA. The results of this study suggests thatthe LC/ESI-ITMS is a promising method for the analysis of BMAA in cyanobacterial matrices.