[Immunobiological characteristics of Mycoplasma hyopneumoniae antigens and the diagnosis of infection caused by this organism].

Abstract

The paper presents the developed variants of enzyme immunoassay (EIA) using two M. hyopneumoniae (R-14 strain) antigens obtained by biomass freezing and thawing and two Tween-dissolved antigens by gel chromatography. To identify the cultures isolated, an indirect immunofluorescence reaction was proposed by employing agar blocks. The developed methods made it possible to identify 23 bovine-isolated cultures as M. hyopneumoniae antigens. The latter were detected by EIA in diagnostically significant titers in serum and colostrum samples from 2 swine-breeding farms. A negative result was obtained in the respiratory mycoplasmosis-free farms.