Macro and micro-structural study on Aspergillus parasiticus inoculated in peanut kernels treated with gamma radiation (Cs137)

Valéria Barbosa Borges, Maria Antonieta Peixoto Gimenes Couto, M. C. L. Souza, Á. Moraes, Marta Maria Braga Baptista Soares Xavier
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引用次数: 1

Abstract

This study investigated the micro and macro-morphology of Aspergillus parasiticus , CMT 00064, inoculated into peanut kernels, after gamma irradiation with Cs137. Plates containing kernels inoculated with the fungal strain were incubated in a BOD germination chamber at 25 o C for 5 days. These plates were irradiated at the dose rate of 1.6 kGy/min on the sixth day of incubation. The absorbed doses were: 0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5 and 8.0 kGy. The observed inactivation doses ranged from 4.5 to 8.0 kGy between days 1, 7 and 15 after irradiation. Fungus growth was observed 15 days after irradiation in the plates irradiated between 0 and 7.5 kGy demonstrating that the dose of 8.0 kGy could eliminate the fungus. Subcultures in nutrient media of isolates irradiated at 7.5 kGy recovered its growth in 15 days. Kernels irradiated with 6.5 kGy grew without metula indicating elimination. Colonies that grown after irradiation up 6.0 kGy were biseriate. However, after irradiation with 6.5 kGy they were uniseriate. The survival rates of A. parasiticus , in irradiated substrate peanuts, decreases with increasing absorbing doses of ionizing radiation.
辐照(Cs137)处理花生籽粒中寄生曲霉的宏观和微观结构研究
本研究以Cs137为辐射源,研究了花生寄生曲霉(Aspergillus parasiticus, CMT 00064)经γ射线照射后的微观和宏观形态。在25℃的BOD萌发室中,将接种了真菌菌株的含粒板培养5天。在培养第6天,以1.6 kGy/min的剂量率照射这些板。吸收剂量分别为:0、3.5、4.0、4.5、5.0、5.5、6.0、6.5、7.0、7.5和8.0 kGy。在辐照后第1、7和15天,观察到的失活剂量范围为4.5至8.0 kGy。在0 ~ 7.5 kGy辐照15天后观察到真菌的生长,表明8.0 kGy的剂量可以消除真菌。在7.5 kGy辐照的培养基中,继代培养15天后恢复生长。在6.5 kGy的辐照下,籽粒生长时未出现表明消除的基质。辐照强度为6.0 kGy后生长的菌落为双菌落。但在6.5 kGy辐照后,它们是单一的。在辐照花生基质中,随电离辐射吸收剂量的增加,寄生蜂的存活率降低。
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