The influence of stromal cells on the MTT assay (I)--In vitro chemosensitivity of the tumor and stromal cells to mitomycin C.

A Suto
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Abstract

To clarify the influence of stromal cells on the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide assay (MTT assay), a gastric carcinoma cell line (KATO-III) and a human fibroblast cell line (IMR-90) were subjected to a colorimetric assay, in which the chemosensitivity KATO-III was found to be highly sensitive to mitomycin C at 10 micrograms/ml, whereas IMR-90 was insensitive to mitomycin C at the same concentration. When the mixtures of these two cell lines were tested by the assay, a mixture of more than 25 per cent stromal cells reduced the sensitivity of KATO-III to mitomycin C. This suggested that the stromal cells in fresh surgical specimens might reduce the apparent sensitivity of the tumor cells.

基质细胞对MTT测定的影响(I)——肿瘤和基质细胞对丝裂霉素C的体外化学敏感性
为了阐明基质细胞对3-(4,5-二甲基-2-噻唑基)-2,5-二苯基- 2h四唑溴化试验(MTT试验)的影响,对胃癌细胞系(KATO-III)和人成纤维细胞系(IMR-90)进行了比色试验,发现KATO-III的化学敏感性对10微克/毫升丝裂霉素C高度敏感,而IMR-90对相同浓度丝裂霉素C不敏感。当对这两种细胞系的混合物进行检测时,超过25%的基质细胞混合物降低了KATO-III对丝裂霉素c的敏感性,这表明新鲜手术标本中的基质细胞可能降低了肿瘤细胞的表观敏感性。
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