Potensi Multiherbal Daun Benalu Teh (Scurrula oortiana), Batang Pasak Bumi (Eurycoma longifolia Jack) dan Batang Pulai (Alstonia scholaris) Sebagai Antioksidan

Abstract

Penelitian potensi aktivitas antioksidan kombinasi multi ekstrak benalu teh, batang pasak bumi, dan batang pulai perbedaan metode ekstraksi dan jenis pelarut serta analisis senyawa kimia yang mempunyai potensi aktivitas antioksidan paling tinggi. Ekstraksi dilakukan dengan cara maserasi dalam etanol 96 % dan refluks dengan akuades. Kombinasi gabungan ekstrak dan gabungan herbal dibuat dengan ratio (1:1:1), (1:1:2), (1:2:1), dan (2:1:1) kemudian diuji aktivitas antioksidan dengan reagen DPPH (2,2-Difenil-1-pikrilhidrazil). Kombinasi multi-ekstrak dan multi-herbal yang memiliki daya hambat paling tinggi diidentifikasi dengan analisis Kromatografi Gas-Spektrometri Massa (KG-MS). Hasil penelitian menunjukkan bahwa kombinasi multi-ekstrak etanol memiliki aktivitas antioksidan sebesar IC 50 19,18 ppm dan kombinasi multi-herbal etanol sebesar IC 50 17,98 ppm. Hasil analisa KG-MS pada ekstrak etanol multi-herbal menunjukkan bahwa senyawa kimia aktif yang berperan ABSTRACT: The leaves of tea parasite (Scurrula oortiana), stems of pasak bumi (Eurycoma longifolia Jack), and stems of pulai (Alstonia scholaris) have been found to be of benefit as an antioxidant. This research aims to know the potential antioxidant activity of combination of multi-extract and multi-herbal tea parasite leaves, pasak bumi stems, and pulai stems based on different extraction methods and variation of solvents and to analysis of chemical compounds that have the highest potential antioxidant activity. Extraction of bioactive compounds was carried out by maceration method extraction using 96% ethanol and reflux using distilled water. The combination of multi-extracts and multi-herbs was made in a ratio of (1:1:1), (1:1:2), (1:2:1), and (2:1:1) and then tested for antioxidant activity by DPPH reagent (2,2-Diphenyl-1-picrylhydrazyl). The combination of multi-extracts and multi-herbs that had the highest inhibition value was identified by gas chromatography-mass spectra (GC-MS) analysis.nThe results showed that the ethanol multi-extract combination had an antioxidant activity of IC 50 19.18 ppm and the multiherbal ethanol combination IC 50 17.98 ppm. Gas Chromatography-mass spectra analysis on multi-herbal ethanol extracts showed that the active chemical compounds that act as antioxidants in a 2:1:1 combination were caffeine.