Intestinal microbiota composition with probiotic potential of three species of the genus Chirostoma

Abstract

The aim of this research was to identify the microbiota that was found in C, jordani, C. humboldtianum and C. estor to obtain those strains with probiotic capacity and can be used in Chirostoma sp. culture. Pre-adult stages were obtained for dissection and gain an intestinal tissue portion and get pure strains throughout consecutive reseeding in NHI and TSA agar plates. Then proceeded to the molecular identification with RNAr 16S sequencing gen and establish their probiotic capacity growing them in acid pH, bile salts, pathogen inhibition to Aeromonas hydrophila and Vibrio fluvialisin vitro and with antibiotics according to FAO (2006) and Balcazar et al . (2006) techniques. Results shown that analyzed Chirostoma sp. from intestinal tract were dominated by Proteobacteria, Actinobacteria and Firmicutes identifying 60 strains, 40% of them were from C. jordani ; 30% from C. humboldtianum and 20% of strains were from C. estor . The three analyzed species share some bacterial groups even if they were grown in different environmental conditions. The common species strains were Lactobacillus sp., L.crispatus , Bacillus subtilis , B. sp., Aeromonashydrophila, A. hydrophila caviae, Vibrio fluvialis and Pseudomonaluteola . The only bacterial species that resisted stress experiments, correspond to Bacillus sp. genus represented by Bacillus subtilis , Bacillus sp. and Bacillus laterodporus , so they are good probiotics candidates for culture of Chirostoma sp. genus.