Significance of Expression of Cyclin D as an Early Indicator in Dysplastic Transformation of Oral Mucosa in Tobacco Users

Abstract

OBJECTIVES: To observe the expression of Cyclin D in transition of normal oral mucosa to dysplastic lesions and to find out the possible association of immunostaining in normal oral mucosa and different grades of oral dysplasiaMETHODOLOGY: In this cross sectional analytical study, total of 120 diagnosed paraffin embedded blocks were included comprising of 60 samples of normal oral mucosa (Group 1) and 60 cases of various grades of oral epithelial dysplastic lesions(Group 2). Patient's were age, was on Cyclin in of to dysplasia.RESULTS: In Group 1, 45/60 (75%) patients were negative for Cyclin D. In Group 2, 40/60 (66%) were negative for Cyclin D. We found non significant association for Cyclin D staining in transition of normal oral mucosa to low grade lesions. But significant association was found in Cyclin D positivity in transition from normal mucosa to high grade dysplastic lesions.CONCLUSION: We found no of Cyclin D marker between normal and early dysplastic lesions, but the expression for Cyclin D was shown to be increased with increasing irreversible grades of dysplasia ie: from normal oralmucosa to severe dysplasia.